C57BL/6JCya-Nrip2em1flox/Cya
Common Name:
Nrip2-flox
Product ID:
S-CKO-12532
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nrip2-flox
Strain ID
CKOCMP-60345-Nrip2-B6J-VA
Gene Name
Product ID
S-CKO-12532
Gene Alias
NIX1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nrip2em1flox/Cya mice (Catalog S-CKO-12532) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000120405
NCBI RefSeq
NM_001162858
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Nrip2, Nuclear Receptor-Interacting Protein 2, is involved in modulating important biological pathways. It has been associated with the Wnt pathway, which is crucial in various cellular processes such as cell proliferation, differentiation, and self-renewal [1].
In colorectal cancer initiating cells, NRIP2 is significantly up-regulated. Knockdown of NRIP2 attenuated Wnt activity, indicating its positive regulation of the Wnt pathway [1]. In podocyte injury, NRIP2 binds β-catenin and stabilizes it through the ubiquitin proteasomal pathway. Genetic knockout of Nrip2 in an adriamycin nephropathy mouse model ameliorated podocyte injury, glomerulosclerosis, and proteinuria by inhibiting β-catenin activation [2].
In conclusion, Nrip2 plays important roles in the Wnt pathway regulation and podocyte injury. The use of gene knockout mouse models, like the Nrip2 knockout in the adriamycin nephropathy model, has been instrumental in revealing its functions in disease-related processes such as colorectal cancer and podocyte-associated kidney diseases.
References：
1. Wen, Zhenzhen, Pan, Tianhui, Yang, Saisai, Mao, Jianshan, Zhu, Yongliang. 2017. Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting RORβ. In Molecular cancer, 16, 20. doi:10.1186/s12943-017-0590-2. https://pubmed.ncbi.nlm.nih.gov/28137278/
2. Hou, Qing, Le, Weibo, Kan, Shuyan, Liu, Zhihong, Chen, Zhaohong. 2021. Nuclear Receptor Interacting Protein-2 Mediates the Stabilization and Activation of β-Catenin During Podocyte Injury. In Frontiers in cell and developmental biology, 9, 781792. doi:10.3389/fcell.2021.781792. https://pubmed.ncbi.nlm.nih.gov/35004680/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen