C57BL/6JCya-Il21rem1flox/Cya
Common Name:
Il21r-flox
Product ID:
S-CKO-12542
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il21r-flox
Strain ID
CKOCMP-60504-Il21r-B6J-VA
Gene Name
Product ID
S-CKO-12542
Gene Alias
NILR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il21rem1flox/Cya mice (Catalog S-CKO-12542) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033000
NCBI RefSeq
NM_021887
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Il21r, the interleukin-21 receptor, is a crucial component in the immune-related cytokine signaling pathway. It binds to interleukin-21, a cytokine mainly produced by CD4 T cells. This interaction is involved in multiple biological processes such as promoting effector functions and memory differentiation of CD8 T cells, and is associated with the regulation of the Th2 immune response, as well as playing roles in cell survival and differentiation in the immune system [2,3,4].
In breast cancer, IL21R hypomethylation was identified as a potential biomarker to distinguish benign and malignant breast tumours. BC patients showed significant IL21R hypomethylation compared to those with benign tumours, and this hypomethylation was more pronounced in ER-negative, PR-negative, and triple-negative tumours. The methylation of IL21R had efficient discriminatory power in differentiating between these tumour types [1].
In multiple myeloma, bone marrow CD14+ cells from patients with multiple myeloma over-expressed IL21R, which was independent of interleukin-21 presence, and this over-expression increased osteoclast formation. Inhibition of interleukin-21 receptor signaling suppressed osteoclast differentiation from these cells [5].
In metabolic dysfunction-associated steatohepatitis-driven hepatocellular carcinoma, IL-21R signaling promoted the disease by inducing immunosuppressive IgA+ B cells. High IL-21R expression in HCC patients was associated with poor relapse-free survival, advanced TNM stage, and severe steatosis, and ablation of IL-21R impeded hepatocarcinogenesis [6].
In conclusion, Il21r plays essential roles in immune-related processes and is associated with various diseases including breast cancer, multiple myeloma, and hepatocellular carcinoma. Research using different models has revealed its significance in disease-related biological mechanisms, such as its potential as a biomarker in breast cancer diagnosis and its role in promoting tumour-related processes in multiple myeloma and hepatocellular carcinoma. These findings contribute to our understanding of disease pathogenesis and may offer new directions for disease diagnosis and treatment.
References：
1. Zang, Zishan, Yin, Yifei, Liu, Chunlan, Li, Hong, Yang, Rongxi. 2024. IL21R hypomethylation as a biomarker for distinguishing benign and malignant breast tumours. In Epigenetics, 19, 2352683. doi:10.1080/15592294.2024.2352683. https://pubmed.ncbi.nlm.nih.gov/38723244/
2. Ren, Heather M, Lukacher, Aron E, Rahman, Ziaur S M, Olsen, Nancy J. 2021. New developments implicating IL-21 in autoimmune disease. In Journal of autoimmunity, 122, 102689. doi:10.1016/j.jaut.2021.102689. https://pubmed.ncbi.nlm.nih.gov/34224936/
3. Carneiro, Valdirene Leão, da Silva, Hugo Bernardino Ferreira, Queiroz, Gerson de Almeida, Cooper, Philip J, Figueiredo, Camila Alexandrina. 2021. WSB1 and IL21R Genetic Variants Are Involved in Th2 Immune Responses to Ascaris lumbricoides. In Frontiers in immunology, 12, 622051. doi:10.3389/fimmu.2021.622051. https://pubmed.ncbi.nlm.nih.gov/33692795/
4. Sun, Weiwei, Gao, Ce, Hartana, Ciputra Adijaya, Yu, Xu G, Lichterfeld, Mathias. 2023. Phenotypic signatures of immune selection in HIV-1 reservoir cells. In Nature, 614, 309-317. doi:10.1038/s41586-022-05538-8. https://pubmed.ncbi.nlm.nih.gov/36599977/
5. Bolzoni, Marina, Ronchetti, Domenica, Storti, Paola, Aversa, Franco, Giuliani, Nicola. 2017. IL21R expressing CD14+CD16+ monocytes expand in multiple myeloma patients leading to increased osteoclasts. In Haematologica, 102, 773-784. doi:10.3324/haematol.2016.153841. https://pubmed.ncbi.nlm.nih.gov/28057743/
6. Xie, Ying, Huang, Yu, Li, Zhi-Yong, Yin, Zhinan, Lin, Xue-Jia. 2024. Interleukin-21 receptor signaling promotes metabolic dysfunction-associated steatohepatitis-driven hepatocellular carcinoma by inducing immunosuppressive IgA+ B cells. In Molecular cancer, 23, 95. doi:10.1186/s12943-024-02001-2. https://pubmed.ncbi.nlm.nih.gov/38720319/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen