C57BL/6JCya-Ciao2bem1flox/Cya
Common Name
Ciao2b-flox
Product ID
S-CKO-14203
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-68523-Ciao2b-B6J-VA
When using this mouse strain in a publication, please cite “Ciao2b-flox Mouse (Catalog S-CKO-14203) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ciao2b-flox
Strain ID
CKOCMP-68523-Ciao2b-B6J-VA
Gene Name
Product ID
S-CKO-14203
Gene Alias
1110019N10Rik, Fam96b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000164884
NCBI RefSeq
NM_026753
Target Region
Exon 1~5
Size of Effective Region
~2.1 kb
Overview of Gene Research
Ciao2b is a subunit of the cytosolic iron-sulfur (Fe-S) assembly (CIA) targeting complex. The CIA pathway is essential for inserting Fe-S clusters into cytosolic and nuclear client proteins involved in key cellular functions like DNA replication and repair [1].
In Caenorhabditis elegans, deletion of CIAO-2B led to sequestration of DOG-1 (homologous to human BRIP1) in the cytoplasm, increasing DNA damage sensitivity. This indicates that CIAO-2B is vital for DOG-1's stability and repair functions, highlighting its role in maintaining genome integrity [2].
In conclusion, CIAO2B is crucial for the proper functioning of the CIA pathway, especially in processes related to DNA metabolism and genome integrity. The study in C. elegans provides insights into how CIAO2B deficiency can lead to DNA damage-related issues, potentially offering a model for understanding similar mechanisms in diseases associated with DNA repair defects.
References：
1. Kassube, Susanne A, Thomä, Nicolas H. 2020. Structural insights into Fe-S protein biogenesis by the CIA targeting complex. In Nature structural & molecular biology, 27, 735-742. doi:10.1038/s41594-020-0454-0. https://pubmed.ncbi.nlm.nih.gov/32632277/
2. Li, Xiao, Perdomo, Ivette Maria Menendez, Rodrigues Alves Barbosa, Victoria, Diao, Catherine, Tarailo-Graovac, Maja. . The critical role of the iron-sulfur cluster and CTC components in DOG-1/BRIP1 function in Caenorhabditis elegans. In Nucleic acids research, 52, 9586-9595. doi:10.1093/nar/gkae617. https://pubmed.ncbi.nlm.nih.gov/39011897/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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