C57BL/6JCya-Cysltr2em1flox/Cya
Common Name:
Cysltr2-flox
Product ID:
S-CKO-14729
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cysltr2-flox
Strain ID
CKOCMP-70086-Cysltr2-B6J-VA
Gene Name
Product ID
S-CKO-14729
Gene Alias
2300001H05Rik; CYSLT2R; Cltr2; Cyslt2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cysltr2em1flox/Cya mice (Catalog S-CKO-14729) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044664
NCBI RefSeq
NM_133720
Target Region
Exon 6
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Cysltr2, encoding the G protein-coupled receptor cysteinyl-leukotriene receptor 2, is involved in multiple biological functions. Cysteinyl leukotrienes (CysLTs) can bind to CysLTR2, activating an inflammatory response. It is part of signaling pathways related to inflammation and may also be involved in G-protein-coupled receptor-mediated signaling. Understanding its function is crucial for diseases related to inflammation and abnormal cell growth [3].
In a study on atherosclerosis, genetic or pharmacological inhibition of Cysltr2 alleviated ceramide-induced atherosclerosis aggravation, suggesting that Cysltr2 plays a role in the process of ceramide-aggravated atherosclerosis. In CYSLTR2 -mutant uveal melanoma, the CysLTR2 -L129Q receptor is a constitutively active mutant strongly driving Gq/11 signaling pathways while escaping β -arrestin-mediated downregulation [1,2].
In conclusion, Cysltr2 is important in inflammation-related signaling and disease development. Its inhibition can alleviate atherosclerosis, and in uveal melanoma, its mutant form has distinct signaling characteristics. Gene -knockout or -knockdown models in mice for Cysltr2 have been valuable in revealing its role in these disease conditions, providing insights for potential therapeutic strategies.
References:
1. Zhang, Siting, Lin, Hui, Wang, Jiale, Sun, Jinpeng, Kong, Wei. 2025. Sensing ceramides by CYSLTR2 and P2RY6 to aggravate atherosclerosis. In Nature, 641, 476-485. doi:10.1038/s41586-025-08792-8. https://pubmed.ncbi.nlm.nih.gov/40049228/
2. Ceraudo, Emilie, Horioka, Mizuho, Mattheisen, Jordan M, Sakmar, Thomas P, Huber, Thomas. 2020. Direct evidence that the GPCR CysLTR2 mutant causative of uveal melanoma is constitutively active with highly biased signaling. In The Journal of biological chemistry, 296, 100163. doi:10.1074/jbc.RA120.015352. https://pubmed.ncbi.nlm.nih.gov/33288675/
3. Dos S Jesus, Talita, Dos S Costa, Ryan, Alcântara-Neves, Neuza Maria, Barreto, Maurício L, Figueiredo, Camila Alexandrina. 2019. Variants in the CYSLTR2 are associated with asthma, atopy markers and helminths infections in the Brazilian population. In Prostaglandins, leukotrienes, and essential fatty acids, 145, 15-22. doi:10.1016/j.plefa.2019.05.003. https://pubmed.ncbi.nlm.nih.gov/31126515/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen