C57BL/6JCya-Gprc5cem1flox/Cya
Common Name:
Gprc5c-flox
Product ID:
S-CKO-14790
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gprc5c-flox
Strain ID
CKOCMP-70355-Gprc5c-B6J-VA
Gene Name
Product ID
S-CKO-14790
Gene Alias
1110028I06Rik; 3200002M13Rik; Raig3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gprc5cem1flox/Cya mice (Catalog S-CKO-14790) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053361
NCBI RefSeq
NM_001110337
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Gprc5c, an orphan G protein-coupled receptor belonging to the class C GPCR family, has diverse functions. It is involved in regulating hematopoietic stem cell (HSC) dormancy, and its associated pathways may include those related to NF-κB activation, cilia composition regulation, smooth muscle contraction, and saccharide sensing. It plays a significant role in various biological processes and diseases [4,5].
In acute myeloid leukemia (AML), high Gprc5c levels in patient cohorts correlated with poorer survival. Ectopic Gprc5c expression increased AML aggression through NF-κB activation, altering metabolism with increased intracellular branched-chain amino acids (BCAAs). Loss of Gprc5c reversed this onco-metabolic profile and abrogated leukemia-initiating potential [1].
In olfactory neurons, Gprc5c deficiency altered sensory cilia structure, increased ciliary layer thickness, and changed the localization of olfactory signalling proteins, leading to increased neuronal activity and improved smell-based food detection in knockout mice [2].
In smooth muscle cells, tamoxifen-inducible, SMC-specific Gprc5c knockout mice showed reduced angiotensin II-dependent calcium mobilization, contraction, and arterial hypertension. Knockdown of Gprc5c in human aortic SMC diminished Ang II-dependent inositol phosphate production and myosin light chain phosphorylation [3].
In the kidney, Gprc5c KO mice had altered acid-base homeostasis, with lower blood pH and higher urine pH. Gprc5c internalization was triggered by alkaline extracellular pH, and it increased Na+/H+ exchanger 3 (NHE3) activity at alkaline pH, which was reduced in Gprc5c KO mice [6].
In summary, Gprc5c has essential functions in multiple biological processes. Its role in HSC dormancy, AML, olfactory function, smooth muscle contraction, and renal acid-base regulation has been revealed through gene knockout mouse models. These findings contribute to understanding the mechanisms of related diseases and may provide potential therapeutic targets for diseases such as AML and arterial hypertension.
References：
1. Zhang, Yu Wei, Velasco-Hernandez, Talia, Mess, Julian, Menendez, Pablo, Cabezas-Wallscheid, Nina. . GPRC5C drives branched-chain amino acid metabolism in leukemogenesis. In Blood advances, 7, 7525-7538. doi:10.1182/bloodadvances.2023010460. https://pubmed.ncbi.nlm.nih.gov/37639313/
2. Bhat, Sneha, Dietz, André, Senf, Katja, Westermann, Martin, Neuhaus, Eva Maria. 2023. GPRC5C regulates the composition of cilia in the olfactory system. In BMC biology, 21, 292. doi:10.1186/s12915-023-01790-0. https://pubmed.ncbi.nlm.nih.gov/38110903/
3. Wang, Tianpeng, Shao, Jingchen, Kumar, Shamit, Offermanns, Stefan, Wettschureck, Nina. 2024. Orphan GPCR GPRC5C Facilitates Angiotensin II-Induced Smooth Muscle Contraction. In Circulation research, 134, 1259-1275. doi:10.1161/CIRCRESAHA.123.323752. https://pubmed.ncbi.nlm.nih.gov/38597112/
4. Zhang, Yu Wei, Mess, Julian, Aizarani, Nadim, Grün, Dominic, Cabezas-Wallscheid, Nina. 2022. Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells. In Nature cell biology, 24, 1038-1048. doi:10.1038/s41556-022-00931-x. https://pubmed.ncbi.nlm.nih.gov/35725769/
5. Kawabata, Yuko, Takai, Shingo, Sanematsu, Keisuke, Jimi, Eijiro, Shigemura, Noriatsu. 2023. The G protein-coupled receptor GPRC5C is a saccharide sensor with a novel 'off' response. In FEBS letters, 597, 2006-2016. doi:10.1002/1873-3468.14695. https://pubmed.ncbi.nlm.nih.gov/37418589/
6. Rajkumar, Premraj, Cha, Boyoung, Yin, Jianyi, Donowitz, Mark, Pluznick, Jennifer L. 2018. Identifying the localization and exploring a functional role for Gprc5c in the kidney. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32, 2046-2059. doi:10.1096/fj.201700610RR. https://pubmed.ncbi.nlm.nih.gov/29196502/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen