C57BL/6JCya-Derl3em1flox/Cya
Common Name:
Derl3-flox
Product ID:
S-CKO-14802
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Derl3-flox
Strain ID
CKOCMP-70377-Derl3-B6J-VA
Gene Name
Product ID
S-CKO-14802
Gene Alias
1810006I20Rik; 1810063P04Rik; IZP6; derlin-3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Derl3em1flox/Cya mice (Catalog S-CKO-14802) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009236
NCBI RefSeq
NM_024440
Target Region
Exon 1~7
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
DERL3, also known as Derlin-3, is a key gene in the endoplasmic reticulum-associated protein degradation pathway. It is involved in the degradation of misfolded lumenal glycoproteins induced by endoplasmic reticulum (ER) stress [7].
DERL3 has been studied in multiple disease contexts. In colorectal cancer, its low expression in cancer tissues is linked to a high rate of lymphatic and distant metastasis. Overexpression of DERL3 in HT29 cells suppresses their proliferative, migratory, and invasive capacities, likely through negatively regulating MYCN level [1].
In gastric cancer, DERL3 acts as a tumor suppressor, with its promoter methylation correlated with tumor clinical features. Genes co-expressed with DERL3 are involved in adaptive immune response, T cell activation, etc. [3].
In nasopharyngeal carcinoma, Epstein-Barr virus latent membrane protein 1 represses DERL3 via DNA methylation, and DERL3 knockdown leads to increased cell proliferation, migration, and invasion [5].
In lung adenocarcinoma, DERL3 is upregulated in carcinoma tissues, and high-expressed patients have a worse response to chemotherapy and immunotherapy. It is positively correlated with B cell activation and unfolded protein response (UPR) [6].
In renal clear cell carcinoma, elevated DERL3 expression indicates a poor prognosis [4].
In rheumatoid arthritis, DERL3 is up-regulated in fibroblast-like synoviocytes of RA patients, and TNF-α-induced DERL3 expression promotes inflammation through activating the NF-κB signaling pathway [2].
In conclusion, DERL3 plays crucial roles in various biological processes and diseases. In cancer, it often acts as a tumor suppressor or is associated with prognosis. In rheumatoid arthritis, it contributes to inflammation. Studies on DERL3 help understand disease mechanisms and may provide potential therapeutic targets.
References：
1. Yu, Fubing, Mou, Bo, Sheng, Juan, Qin, Xiangxin, Yu, Jiaman. 2020. DERL3 suppresses colorectal cancer metastasis through negatively regulating MYCN level. In Minerva medica, 114, 316-322. doi:10.23736/S0026-4806.20.06657-4. https://pubmed.ncbi.nlm.nih.gov/32538588/
2. Geng, Manman, Xu, Ke, Meng, Liesu, Zhu, Wenhua, Lu, Shemin. 2020. Up-regulated DERL3 in fibroblast-like synoviocytes exacerbates inflammation of rheumatoid arthritis. In Clinical immunology (Orlando, Fla.), 220, 108579. doi:10.1016/j.clim.2020.108579. https://pubmed.ncbi.nlm.nih.gov/32866644/
3. Li, Yongyuan, Liu, Hongjie, Chen, Hekai, Cai, Xuejun, He, Xianghui. 2019. DERL3 functions as a tumor suppressor in gastric cancer. In Computational biology and chemistry, 84, 107172. doi:10.1016/j.compbiolchem.2019.107172. https://pubmed.ncbi.nlm.nih.gov/31862624/
4. Fan, Weining, Ran, Lingxiang, Wang, Lexin, Chi, Hao. 2024. The elevated DERL3 expression indicates poor prognosis in renal clear cell carcinoma. In Asian journal of surgery, , . doi:10.1016/j.asjsur.2024.06.115. https://pubmed.ncbi.nlm.nih.gov/39034257/
5. Kondo, Satoru, Okabe, Atsushi, Nakagawa, Takuya, Yoshizaki, Tomokazu, Kaneda, Atsushi. 2022. Repression of DERL3 via DNA methylation by Epstein-Barr virus latent membrane protein 1 in nasopharyngeal carcinoma. In Biochimica et biophysica acta. Molecular basis of disease, 1869, 166598. doi:10.1016/j.bbadis.2022.166598. https://pubmed.ncbi.nlm.nih.gov/36372158/
6. Lin, Lanlan, Lin, Guofu, Lin, Hai, Xu, Yuan, Zeng, Yiming. 2022. Integrated profiling of endoplasmic reticulum stress-related DERL3 in the prognostic and immune features of lung adenocarcinoma. In Frontiers in immunology, 13, 906420. doi:10.3389/fimmu.2022.906420. https://pubmed.ncbi.nlm.nih.gov/36275646/
7. Lopez-Serra, Paula, Marcilla, Miguel, Villanueva, Alberto, Albar, Juan Pablo, Esteller, Manel. 2014. A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect. In Nature communications, 5, 3608. doi:10.1038/ncomms4608. https://pubmed.ncbi.nlm.nih.gov/24699711/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen