C57BL/6JCya-Ces2fem1flox/Cya
Common Name:
Ces2f-flox
Product ID:
S-CKO-15251
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ces2f-flox
Strain ID
CKOCMP-71903-Ces2f-B6J-VA
Gene Name
Product ID
S-CKO-15251
Gene Alias
2310038E17Rik; A430088E12
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ces2fem1flox/Cya mice (Catalog S-CKO-15251) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000076384
NCBI RefSeq
NM_001079865
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ces2f, a member of the carboxylesterase 2 (Ces2) family, is a phase I enzyme. Carboxylesterases play a significant role in xenobiotic clearance and lipid metabolism [1]. Genetic models, such as knockout mouse models, can be valuable for studying its functions.
In a study, E4bp4 -/- mice showed down-regulation of an array of hepatic Ces genes including Ces2f [1]. E4bp4 knockdown in Hepa-1c1c7 cells also reduced the expression of Ces2f [1]. This indicates that E4bp4 is involved in regulating the expression of Ces2f. Moreover, E4bp4 -/- mice had reduced Ces-mediated metabolism and elevated systemic exposure of CPT-11, a Ces substrate, suggesting that changes in Ces2f expression, as part of the overall Ces regulation, impact the metabolism of such substrates [1].
In conclusion, Ces2f, as part of the Ces enzyme family, is crucial for xenobiotic clearance and lipid metabolism. The use of E4bp4 -/- mouse models has revealed that E4bp4 is an important regulator of Ces2f expression, and alterations in Ces2f expression can affect the metabolism of its substrates like CPT-11. This contributes to our understanding of the role of Ces2f in drug metabolism-related processes.
References：
1. Zhao, Mengjing, Zhang, Tianpeng, Yu, Fangjun, Guo, Lianxia, Wu, Baojian. 2018. E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice. In Biochemical pharmacology, 152, 293-301. doi:10.1016/j.bcp.2018.04.005. https://pubmed.ncbi.nlm.nih.gov/29653076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen