C57BL/6JCya-Fbxo47em1flox/Cya
Common Name:
Fbxo47-flox
Product ID:
S-CKO-15605
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fbxo47-flox
Strain ID
CKOCMP-72973-Fbxo47-B6J-VA
Gene Name
Product ID
S-CKO-15605
Gene Alias
2900052P03Rik; Gm1562
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo47em1flox/Cya mice (Catalog S-CKO-15605) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093939
NCBI RefSeq
NM_001081435
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Fbxo47, an F-box protein, is a key component of the centromeric SCF E3 ubiquitin ligase complex. It is involved in multiple meiotic processes, such as centromere pairing, telomere-inner nuclear envelope integration, and the stability of the synaptonemal complex during meiotic prophase I. These functions are crucial for proper homologous chromosome synapsis, recombination, and gametogenesis. Mouse models have been valuable in studying its functions [1,2,3].
In mouse models, knockout of Fbxo47 leads to male infertility. Fbxo47-deficient spermatocytes show defective centromere pairing, incomplete synaptonemal complex formation, unstable telomere attachment, and premature disassembly of the synaptonemal complex. FBXO47 also interacts with proteins like SKP1, TRF1/2, and HORMAD1. It stabilizes SKP1 by down-regulating its ubiquitination, and targets HORMAD1 for polyubiquitination and degradation. Disruption of Fbxo47 destabilizes TRF2 [1,2,3,4].
In summary, Fbxo47 plays essential roles in meiotic processes, especially in ensuring proper chromosome synapsis, recombination, and gamete formation. Mouse knockout models have been instrumental in revealing its functions in male infertility-related meiotic disorders. Additionally, in non-reproductive contexts, it may be involved in cancer-related processes as it is targeted by tRF-3019a in gastric cancer and miRNA-566 in non-small cell lung cancer [2,5,6].
References:
1. Ma, Ani, Yang, Yali, Cao, Lianbao, Chen, Lijun, Zhang, Jian V. 2024. FBXO47 regulates centromere pairing as key component of centromeric SCF E3 ligase in mouse spermatocytes. In Communications biology, 7, 1099. doi:10.1038/s42003-024-06782-6. https://pubmed.ncbi.nlm.nih.gov/39244596/
2. Hua, Rong, Wei, Huafang, Liu, Chao, Li, Wei, Liu, Mingxi. . FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis. In Nucleic acids research, 47, 11755-11770. doi:10.1093/nar/gkz992. https://pubmed.ncbi.nlm.nih.gov/31724724/
3. Tanno, Nobuhiro, Takemoto, Kazumasa, Takada-Horisawa, Yuki, Araki, Kimi, Ishiguro, Kei-Ichiro. 2022. FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis. In iScience, 25, 104008. doi:10.1016/j.isci.2022.104008. https://pubmed.ncbi.nlm.nih.gov/35310947/
4. Guan, Yongjuan, Lin, Huijuan, Leu, N Adrian, Luo, Mengcheng, Wang, P Jeremy. . SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. In Nucleic acids research, 50, 5129-5144. doi:10.1093/nar/gkac304. https://pubmed.ncbi.nlm.nih.gov/35489071/
5. Zhang, Fei, Shi, Jinxin, Wu, Zhonghua, Song, Yongxi, Wang, Zhenning. 2020. A 3'-tRNA-derived fragment enhances cell proliferation, migration and invasion in gastric cancer by targeting FBXO47. In Archives of biochemistry and biophysics, 690, 108467. doi:10.1016/j.abb.2020.108467. https://pubmed.ncbi.nlm.nih.gov/32592804/
6. Lv, Hualiang, Lai, Changchun, Zhao, Wenqu, Song, Yibo. 2022. GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting miRNA-566/F-box protein 47. In Oncology research, 29, 401-409. doi:10.32604/or.2022.025262. https://pubmed.ncbi.nlm.nih.gov/37304650/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen