C57BL/6JCya-Agmatem1flox/Cya
Common Name:
Agmat-flox
Product ID:
S-CKO-16375
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Agmat-flox
Strain ID
CKOCMP-75986-Agmat-B6J-VA
Gene Name
Product ID
S-CKO-16375
Gene Alias
5033405N08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Agmatem1flox/Cya mice (Catalog S-CKO-16375) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038161
NCBI RefSeq
NM_001081408
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
AGMAT, also known as agmatinase, is an enzyme involved in the polyamine synthesis pathway. In the non-classical polyamine synthesis pathway, it hydrolyzes agmatine, which is converted from arginine by arginine decarboxylase, to form putrescine [2]. Polyamines are essential for processes such as cell division, proliferation, and embryonic development [2].
In the context of diseases, in vivo knockdown of translation of mRNAs for both ornithine decarboxylase 1 (ODC1) and AGMAT in sheep during the peri-implantation period of pregnancy led to abnormal conceptuses and a reduced pregnancy rate. This also increased the expression of genes related to polyamine synthesis and transport [4].
In colorectal carcinoma, miR-151a-5p was found to promote cell proliferation and metastasis by targeting AGMAT, and AGMAT was positively associated with miR-151a-5p expression [1].
In pancreatic adenocarcinoma, AGMAT was overexpressed, and its overexpression promoted cell proliferation and metastasis through activation of the TGFβ/Smad pathway [5].
In esophageal adenocarcinoma, AGMAT was overexpressed at the protein level with a proposed EAC-selective, post-transcriptional mechanism of regulation of protein abundance [3].
In rats, overexpression of AGMAT in the ventral hippocampus elicited depressive-and anxiety-like behaviors, while knockdown of AGMAT exhibited antidepressant and anxiolytic effects in a chronic restraint stress model [6].
In conclusion, AGMAT plays a crucial role in polyamine synthesis and is involved in various biological processes and disease conditions. Studies in animal models, such as in sheep during pregnancy and rats under stress, as well as in cancer research, have revealed its significance in embryonic development, cancer progression, and psychiatric disorders. Understanding AGMAT's functions provides insights into the underlying mechanisms of these biological processes and diseases, potentially leading to new therapeutic strategies.
References:
1. Xie, Yaya, Zhang, Yue, Liu, Xianju, Chen, Jinlian, Zhang, Xingxing. 2023. miR‑151a‑5p promotes the proliferation and metastasis of colorectal carcinoma cells by targeting AGMAT. In Oncology reports, 49, . doi:10.3892/or.2023.8487. https://pubmed.ncbi.nlm.nih.gov/36704851/
2. Lenis, Yasser Y, Elmetwally, Mohammed A, Maldonado-Estrada, Juan G, Bazer, Fuller W. 2017. Physiological importance of polyamines. In Zygote (Cambridge, England), 25, 244-255. doi:10.1017/S0967199417000120. https://pubmed.ncbi.nlm.nih.gov/28587687/
3. O'Neill, J Robert, Yébenes Mayordomo, Marcos, Mitulović, Goran, Hupp, Ted, Alfaro, Javier Antonio. 2024. Multi-Omic Analysis of Esophageal Adenocarcinoma Uncovers Candidate Therapeutic Targets and Cancer-Selective Posttranscriptional Regulation. In Molecular & cellular proteomics : MCP, 23, 100764. doi:10.1016/j.mcpro.2024.100764. https://pubmed.ncbi.nlm.nih.gov/38604503/
4. Lenis, Yasser Y, Elmetwally, Mohammed A, Tang, Wanjin, Wu, Guoyao, Bazer, Fuller W. 2017. Functional roles of agmatinase during the peri-implantation period of pregnancy in sheep. In Amino acids, 50, 293-308. doi:10.1007/s00726-017-2515-1. https://pubmed.ncbi.nlm.nih.gov/29196820/
5. Zhang, Yue, Cao, Lijun, Xie, Yaya, Zhang, Xingxing, Chen, Jinlian. 2022. Agmatinase facilitates the tumorigenesis of pancreatic adenocarcinoma through the TGFβ/Smad pathway. In Experimental and therapeutic medicine, 24, 490. doi:10.3892/etm.2022.11417. https://pubmed.ncbi.nlm.nih.gov/35837051/
6. Yan, Shi, Xu, Chang, Yang, Mengli, Tong, Zhiqian, Cai, Xiang. 2023. The expression of agmatinase manipulates the affective state of rats subjected to chronic restraint stress. In Neuropharmacology, 229, 109476. doi:10.1016/j.neuropharm.2023.109476. https://pubmed.ncbi.nlm.nih.gov/36849038/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen