C57BL/6JCya-Polr3hem1flox/Cya
Common Name:
Polr3h-flox
Product ID:
S-CKO-16904
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Polr3h-flox
Strain ID
CKOCMP-78929-Polr3h-B6J-VA
Gene Name
Product ID
S-CKO-16904
Gene Alias
5031409G22Rik; RPC8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Polr3hem1flox/Cya mice (Catalog S-CKO-16904) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023113
NCBI RefSeq
NM_030229
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
POLR3H is a subunit of RNA polymerase III, which transcribes small untranslated RNAs like 5S ribosomal RNA, transfer RNAs, and U6 small nuclear RNA, contributing to RNA maturation and translation. It is associated with pathways related to ovarian development and function, and its study in genetic models can offer insights into its role in biological processes and diseases [2].
In mice, loss-of-function Polr3h mutation caused early embryonic lethality. Homozygous point mutation (Polr3hD50G) in mice led to delayed pubertal development, decreased fertility later in life, decreased expression of ovarian Foxo3a, and lower numbers of primary follicles. These findings suggest that POLR3H is crucial for normal pubertal development and fertility in mice [1].
In conclusion, POLR3H plays an essential role in processes related to pubertal development and fertility as revealed through mouse models. Its study in KO mouse models has provided insights into its role in primary ovarian insufficiency, expanding our understanding of the molecular pathways of ovarian function and human infertility [1].
References：
1. Franca, Monica M, Han, Xingfa, Funari, Mariana F A, Elias, Carol F, Mendonca, Berenice B. . Exome Sequencing Reveals the POLR3H Gene as a Novel Cause of Primary Ovarian Insufficiency. In The Journal of clinical endocrinology and metabolism, 104, 2827-2841. doi:10.1210/jc.2018-02485. https://pubmed.ncbi.nlm.nih.gov/30830215/
2. Lata, Elisabeth, Choquet, Karine, Sagliocco, Francis, Bernard, Geneviève, Teichmann, Martin. 2021. RNA Polymerase III Subunit Mutations in Genetic Diseases. In Frontiers in molecular biosciences, 8, 696438. doi:10.3389/fmolb.2021.696438. https://pubmed.ncbi.nlm.nih.gov/34395528/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen