C57BL/6JCya-Rrbp1em1flox/Cya
Common Name:
Rrbp1-flox
Product ID:
S-CKO-17030
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rrbp1-flox
Strain ID
CKOCMP-81910-Rrbp1-B6J-VA
Gene Name
Product ID
S-CKO-17030
Gene Alias
1700087N07Rik; 5730465C04Rik; ES/130; mKIAA1398; mRRp0; mRRp1.8; mRRp10; mRRp15a; mRRp15b; mRRp16.8; mRRp2; mRRp41; mRRp47; mRRp5.4; p180
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rrbp1em1flox/Cya mice (Catalog S-CKO-17030) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016072
NCBI RefSeq
NM_024281
Target Region
Exon 2
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
RRBP1, also known as ribosome-binding protein 1 or P180, is a ribosome-binding transmembrane protein of the endoplasmic reticulum. It is involved in multiple biological functions.
In normal physiological conditions, it contributes to processes such as local translation in axons, where it binds to mRNAs enriched for axonal membrane proteins and regulates local translation through its role in axonal ER-ribosome interactions [2]. It also plays a part in the regulation of mitophagy. When there is mitochondrial protein import stress (MPIS), RRBP1 relocates to the mitochondrial vicinity, and together with NLRX1, controls the recruitment and lipidation of LC3, a crucial step in mitophagy [1,8].
In disease-related studies, RRBP1 shows potential oncogenic properties. In upper tract urothelial carcinoma (UTUC), gene body hypomethylation of RRBP1 is associated with its high expression, which is related to carcinogenesis, metastasis, and chemoresistance [3]. In bladder cancer, high RRBP1 expression is associated with tumor stage, lymph node metastasis, and shorter overall survival time, and its knockdown inhibits cell migration and invasion [4]. In oral squamous cell carcinoma, RRBP1 up-regulation is related to cisplatin-resistance, and its inhibition restores cisplatin-mediated cell death [5]. In prostate cancer, METTL3 stabilizes RRBP1 mRNA in an m6A-dependent manner, and RRBP1 exerts an oncogenic role [7]. In epithelial ovarian cancer, high RRBP1 expression is related to FIGO stage, histological grade, histological type, and lymph node metastasis, and is an independent predictor of overall survival and disease-free survival [9]. In prostate cancer, RRBP1 expression is significantly higher compared to non-cancerous tissues and is associated with T stage, lymph node metastasis, PSA, and Gleason score, serving as an independent prognostic factor [10]. In bone metastatic cancer, depletion of RRBP1 enhances the osteoblastic phenotype expression, suggesting its role in the bone microenvironment [6].
In conclusion, RRBP1 has essential functions in normal biological processes like local translation and mitophagy. In disease, especially in multiple types of cancers, it is closely associated with tumor malignancy, metastasis, and chemoresistance. Studies on RRBP1, including those using gene-knockout or conditional-knockout models if applicable in the future, help to understand its role in these biological processes and diseases, potentially providing new targets for cancer treatment.
References:
1. Killackey, Samuel A, Bi, Yuntian, Soares, Fraser, Arnoult, Damien, Girardin, Stephen E. 2022. Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1. In Molecular cell, 82, 2815-2831.e5. doi:10.1016/j.molcel.2022.06.004. https://pubmed.ncbi.nlm.nih.gov/35752171/
2. Koppers, Max, Özkan, Nazmiye, Nguyen, Ha H, Hoogenraad, Casper C, Farías, Ginny G. 2024. Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions. In Developmental cell, 59, 2053-2068.e9. doi:10.1016/j.devcel.2024.05.005. https://pubmed.ncbi.nlm.nih.gov/38815583/
3. Luo, Hao-Lun, Liu, Hui-Ying, Chang, Yin-Lun, Huang, Chun-Chieh, Peng, Jei-Ming. 2021. Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma. In International journal of molecular sciences, 22, . doi:10.3390/ijms22168761. https://pubmed.ncbi.nlm.nih.gov/34445467/
4. Wang, Mingshuai, Liu, Sai, Zhou, Bolin, Ping, Hao, Xing, Nianzeng. 2020. RRBP1 is highly expressed in bladder cancer and is associated with migration and invasion. In Oncology letters, 20, 203. doi:10.3892/ol.2020.12066. https://pubmed.ncbi.nlm.nih.gov/32963609/
5. Shriwas, Omprakash, Arya, Rakesh, Mohanty, Sibasish, Nanda, Ranjan K, Dash, Rupesh. 2021. RRBP1 rewires cisplatin resistance in oral squamous cell carcinoma by regulating Hippo pathway. In British journal of cancer, 124, 2004-2016. doi:10.1038/s41416-021-01336-7. https://pubmed.ncbi.nlm.nih.gov/33762722/
6. Chen, Rui, Wang, Yue, Xu, Yang, Xia, Chun, Zhang, Bing. 2022. RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype. In Frontiers in oncology, 12, 1005152. doi:10.3389/fonc.2022.1005152. https://pubmed.ncbi.nlm.nih.gov/36568157/
7. Feng, Yuqing, Li, Zenghui, Zhu, Jinwei, Shi, Junfeng, Zhang, Dingxiao. 2024. Stabilization of RRBP1 mRNA via an m6A-dependent manner in prostate cancer constitutes a therapeutic vulnerability amenable to small-peptide inhibition of METTL3. In Cellular and molecular life sciences : CMLS, 81, 414. doi:10.1007/s00018-024-05418-6. https://pubmed.ncbi.nlm.nih.gov/39367907/
8. Killackey, Samuel A, Bi, Yuntian, Philpott, Dana J, Arnoult, Damien, Girardin, Stephen E. 2022. Mitochondria-ER cooperation: NLRX1 detects mitochondrial protein import stress and promotes mitophagy through the ER protein RRBP1. In Autophagy, 19, 1601-1603. doi:10.1080/15548627.2022.2129763. https://pubmed.ncbi.nlm.nih.gov/36170592/
9. Ma, Jing, Ren, Sujing, Ding, Jing, Ma, Rong, Meng, Fanling. 2019. Expression of RRBP1 in epithelial ovarian cancer and its clinical significance. In Bioscience reports, 39, . doi:10.1042/BSR20190656. https://pubmed.ncbi.nlm.nih.gov/31285390/
10. Li, Tieqiu, Wang, Qianqian, Hong, Xiuqin, Li, Jiahui, Lei, Bin. 2019. RRBP1 is highly expressed in prostate cancer and correlates with prognosis. In Cancer management and research, 11, 3021-3027. doi:10.2147/CMAR.S186632. https://pubmed.ncbi.nlm.nih.gov/31118771/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen