C57BL/6JCya-Il23aem1flox/Cya
Common Name:
Il23a-flox
Product ID:
S-CKO-17043
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Il23a-flox
Strain ID
CKOCMP-83430-Il23a-B6J-VA
Gene Name
Product ID
S-CKO-17043
Gene Alias
IL-23; p19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il23aem1flox/Cya mice (Catalog S-CKO-17043) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026449
NCBI RefSeq
NM_031252
Target Region
Exon 1~4
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Il23a, also known as p19, is a subunit of the pro-inflammatory cytokine interleukin-23 (IL-23). IL-23, composed of Il23a and IL-12/23B (p40), drives the differentiation and activation of T helper 17 (Th17) cells, leading to the production of cytokines like IL-17A, IL-17F, IL-6, IL-22, and TNF-α. This cytokine is mainly produced by macrophages and dendritic cells in response to signals. The IL-23/IL-17 pathway is crucial in protective immune responses to certain infections but is also involved in chronic immune-mediated inflammation [2].
In psoriasis, single-cell analysis of human skin identified that CD14+ type 3 dendritic cells in lesional skin increased and co-produced IL1B and Il23a, which are pathological in psoriasis [1]. In the context of cognitive impairment caused by bortezomib exposure, brain endothelial-specific ablation of Il23a ameliorated microglia activation and cognitive dysfunction, suggesting that the endothelial TFEB-STAT3-Il23A axis is critical for initiating bortezomib-mediated aberrant microglial activation [3]. Additionally, in inflammatory bowel disease, higher mucosal Il23a expression was associated with a greater likelihood of achieving remission with ustekinumab treatment, indicating its potential as a biomarker for treatment response prediction [4].
In conclusion, Il23a plays a significant role in the IL-23/IL-17 pathway, which is involved in both normal immune responses and the pathogenesis of various inflammatory diseases such as psoriasis, cognitive impairment related to chemotherapy, and inflammatory bowel disease. Studies using gene-targeted mouse models, like the endothelial-specific Il23a knockout, have provided valuable insights into its role in these disease conditions, highlighting its potential as a therapeutic target or biomarker.
References:
1. Nakamizo, Satoshi, Dutertre, Charles-Antoine, Khalilnezhad, Ahad, Kabashima, Kenji, Ginhoux, Florent. 2021. Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20202345. https://pubmed.ncbi.nlm.nih.gov/34279540/
2. Schinocca, Claudia, Rizzo, Chiara, Fasano, Serena, Ciccia, Francesco, Guggino, Giuliana. 2021. Role of the IL-23/IL-17 Pathway in Rheumatic Diseases: An Overview. In Frontiers in immunology, 12, 637829. doi:10.3389/fimmu.2021.637829. https://pubmed.ncbi.nlm.nih.gov/33692806/
3. Lu, Yaping, Chen, Xiang, Liu, Xiuxiu, Han, Feng, Lu, Ying-Mei. 2023. Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction. In Autophagy, 19, 1803-1820. doi:10.1080/15548627.2022.2162244. https://pubmed.ncbi.nlm.nih.gov/36588318/
4. Nishioka, Kei, Ogino, Haruei, Chinen, Takatoshi, Nakamura, Kazuhiko, Ogawa, Yoshihiro. 2021. Mucosal IL23A expression predicts the response to Ustekinumab in inflammatory bowel disease. In Journal of gastroenterology, 56, 976-987. doi:10.1007/s00535-021-01819-7. https://pubmed.ncbi.nlm.nih.gov/34448069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen