C57BL/6JCya-Hemgnem1flox/Cya
Common Name:
Hemgn-flox
Product ID:
S-CKO-17213
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hemgn-flox
Strain ID
CKOCMP-93966-Hemgn-B6J-VA
Gene Name
Product ID
S-CKO-17213
Gene Alias
4921524M03Rik; EDAG; Hgn; mNDR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hemgnem1flox/Cya mice (Catalog S-CKO-17213) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107764
NCBI RefSeq
NM_053149
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Hemgn, also known as Hemogen in mouse, EDAG in human and RP59 in rat, is a gene encoding a hematopoietic-specific nuclear protein. It is involved in multiple biological processes. In hematopoiesis, it has been linked to the regulation of hematopoietic stem and progenitor cells (HSPCs) through negatively regulating the interferon-γ (IFN-γ) signaling pathway [1]. It also has potential roles in spermatogenesis as a testis-specific isoform exists [2].
In Hemgn-deficient mice, HSPCs show defective engraftment during bone marrow transplantation, with reduced homing, survival, and increased apoptosis. Transcriptome analysis of transplanted Hemgn -/- HSPCs reveals enrichment of gene sets related to IFN-γ signaling. Hemgn -/- HSPCs have enhanced responses to IFN-γ treatment and increased aging over time. Blocking IFN-γ signaling can rescue the engraftment defect of Hemgn -/- HSPCs [1].
In conclusion, Hemgn is a critical regulator for the successful engraftment and reconstitution of HSPCs in mice by negatively regulating IFN-γ signaling. The study of Hemgn knockout mouse models provides insights into its role in HSPC transplantation-related stress, which may help improve conditioning regimens and engraftment during HSPC transplantation [1].
References：
1. Zhao, Ke, Liu, Jin-Fang, Zhu, Ya-Xin, Yang, Xiao-Ming, Li, Chang-Yan. 2021. Hemgn Protects Hematopoietic Stem and Progenitor Cells Against Transplantation Stress Through Negatively Regulating IFN-γ Signaling. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9, e2103838. doi:10.1002/advs.202103838. https://pubmed.ncbi.nlm.nih.gov/34923767/
2. Yang, Li V, Heng, Henry H, Wan, Junmei, Gow, Alexander, Li, Li. . Alternative promoters and polyadenylation regulate tissue-specific expression of Hemogen isoforms during hematopoiesis and spermatogenesis. In Developmental dynamics : an official publication of the American Association of Anatomists, 228, 606-16. doi:. https://pubmed.ncbi.nlm.nih.gov/14648837/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen