C57BL/6JCya-Flot1em1flox/Cya
Common Name:
Flot1-flox
Product ID:
S-CKO-17392
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Flot1-flox
Strain ID
CKOCMP-14251-Flot1-B6J-VA
Gene Name
Product ID
S-CKO-17392
Gene Alias
reggie-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Flot1em1flox/Cya mice (Catalog S-CKO-17392) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001569
NCBI RefSeq
NM_008027
Target Region
Exon 3~8
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Flot1, also known as Flotillin-1, is a scaffold protein of lipid rafts. It is involved in multiple biological processes such as lipid raft protein-dependent or clathrin-independent endocytosis, and the formation of hippocampal synapses. It participates in signaling transduction and vesicular trafficking pathways, playing a significant role in normal physiological functions and disease development [2].
FLOT1 has been found to have a profound impact on cancer progression. In gastric cancer, FLOT1 promotes cell progression and metastasis by interacting with BCAR1 through the ERK signaling pathway [1]. In lung adenocarcinoma, it promotes tumor development, induces epithelial-mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway [5]. In acute myeloid leukemia (AML), increased FLOT1 expression is correlated with poor clinical outcome, and its knockdown inhibits AML cell growth through triggering apoptosis and pyroptosis [4]. In glioblastoma, the SDC1-TGM2-FLOT1-BHMT complex influences the fusion of autophagosomes with lysosomes, determining radiosensitivity [3]. In esophageal cancer, interfering with Circ_0003855 expression, which is related to miR-622 and FLOT1, can inhibit the activity of cancer cells [6]. In ovarian cancer, FLOT1 is a novel serum biomarker, and its mRNA m6A modification can be targeted for inhibition [7].
In conclusion, FLOT1 is crucial in multiple biological processes, especially in cancer-related pathways. The findings from various disease-related studies highlight its potential as a therapeutic target and biomarker. Understanding the role of FLOT1 through functional studies, including gene knockout models if applicable in these disease areas, can provide new insights into disease mechanisms and treatment strategies.
References:
1. Wang, Ran, Huang, Wei, Cai, Kaimei, Chen, Zhikang, Lai, Chen. 2023. FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling. In International journal of biological sciences, 19, 5104-5119. doi:10.7150/ijbs.82606. https://pubmed.ncbi.nlm.nih.gov/37928269/
2. Zhan, Ziqing, Ye, Meng, Jin, Xiaofeng. 2023. The roles of FLOT1 in human diseases (Review). In Molecular medicine reports, 28, . doi:10.3892/mmr.2023.13099. https://pubmed.ncbi.nlm.nih.gov/37772385/
3. Zeng, Liang, Zheng, Wang, Liu, Xinglong, Zhang, Jianghong, Shao, Chunlin. 2023. SDC1-TGM2-FLOT1-BHMT complex determines radiosensitivity of glioblastoma by influencing the fusion of autophagosomes with lysosomes. In Theranostics, 13, 3725-3743. doi:10.7150/thno.81999. https://pubmed.ncbi.nlm.nih.gov/37441590/
4. Mao, Shihui, Qian, Yu, Wei, Wenwen, Sun, Jie, Jin, Jie. 2023. FLOT1 knockdown inhibits growth of AML cells through triggering apoptosis and pyroptosis. In Annals of hematology, 102, 583-595. doi:10.1007/s00277-023-05103-x. https://pubmed.ncbi.nlm.nih.gov/36697954/
5. Zhang, Louqian, Mao, Yuan, Mao, Qixing, Xu, Lin, Wang, Jun. 2019. FLOT1 promotes tumor development, induces epithelial-mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma. In Thoracic cancer, 10, 909-917. doi:10.1111/1759-7714.13027. https://pubmed.ncbi.nlm.nih.gov/30838797/
6. Tian, Jingjing, Hu, Xibao, Zhang, Xinrong. 2024. Circ_0003855 involvement of esophageal cancer progression through miR-622/FLOT1. In Oncology research, 32, 925-931. doi:10.32604/or.2023.042447. https://pubmed.ncbi.nlm.nih.gov/38686057/
7. Guan, Bin, Lu, Qi, Chen, Junyu, Zhang, Lingyun, Xu, Guoxiong. 2025. FLOT1 Is a Novel Serum Biomarker of Ovarian Cancer Targeted by N6-methyladenosine Modification Inhibition. In Cell biology international, 49, 674-691. doi:10.1002/cbin.70015. https://pubmed.ncbi.nlm.nih.gov/40066501/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen