C57BL/6JCya-Metrnem1flox/Cya
Common Name:
Metrn-flox
Product ID:
S-CKO-17408
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Metrn-flox
Strain ID
CKOCMP-70083-Metrn-B6J-VA
Gene Name
Product ID
S-CKO-17408
Gene Alias
1810034B16Rik; EyeLinc16; Hyrac
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Metrnem1flox/Cya mice (Catalog S-CKO-17408) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165838
NCBI RefSeq
NM_133719
Target Region
Exon 1~4
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Metrn, also known as meteorin, is a neurotrophin mainly expressed in the central nervous system, where it regulates glial cell differentiation and promotes axonal elongation [4]. Its homologous protein, METRNL, is associated with various metabolic regulatory pathways such as those related to lipid-induced inflammation, insulin resistance, and fatty acid oxidation through AMP-activated protein kinase (AMPK) or peroxisome proliferator-activated receptor δ (PPARδ)-dependent pathways [3].
In colorectal cancer, METRN is overexpressed, especially in advanced stages and in the MSI (dMMR) subtype, often with BRAF mutations, and is associated with poor prognosis [1]. In glioma, the circular RNA hsa_circ_0037251 generated from METRN promotes cell proliferation, invasion, and migration by sponging miR-1229-3p and regulating mTOR expression [2]. In glioblastoma, low-dose radiation-induced exosomal circ-METRN enhances tumor progression and radioresistance via the miR-4709-3p/GRB14/PDGFRα pathway [5]. In the context of hematopoietic stem/progenitor cell (HSPC) mobilization, Metrn from hypoxic macrophages restrains HSPC mobilization, and macrophage-specific Metrn knockout increases HSPC mobilization [6]. In hyperalgesic priming in mouse models, Metrn is downregulated, and reversing its downregulation can reverse established hyperalgesic priming [7]. In preeclampsia, serum METRN levels are lower in early pregnancy in preeclamptic women compared to healthy pregnant women, and its measurement can assist in predicting preeclampsia [8].
In conclusion, Metrn plays diverse roles in multiple biological processes and disease conditions. Through various model-based research, it has been revealed that Metrn is involved in cancer progression, neural-related pain regulation, and pregnancy-associated disorders. The study of Metrn knockout or conditional knockout mouse models has provided valuable insights into its functions in these specific areas, contributing to a better understanding of the underlying mechanisms and potentially paving the way for new therapeutic strategies.
References：
1. Xu, Xin, Zhang, Chihao, Xia, Yan, Yu, Jiwei. 2019. Over expression of METRN predicts poor clinical prognosis in colorectal cancer. In Molecular genetics & genomic medicine, 8, e1102. doi:10.1002/mgg3.1102. https://pubmed.ncbi.nlm.nih.gov/31859449/
2. Cao, Qinchen, Shi, Yonggang, Wang, Xinxin, Zhai, Guan, Zhang, Mingzhi. 2019. Circular METRN RNA hsa_circ_0037251 Promotes Glioma Progression by Sponging miR-1229-3p and Regulating mTOR Expression. In Scientific reports, 9, 19791. doi:10.1038/s41598-019-56417-8. https://pubmed.ncbi.nlm.nih.gov/31875034/
3. Jung, Tae Woo, Lee, Sung Hoon, Kim, Hyoung-Chun, Shin, Yong Kyoo, Jeong, Ji Hoon. 2018. METRNL attenuates lipid-induced inflammation and insulin resistance via AMPK or PPARδ-dependent pathways in skeletal muscle of mice. In Experimental & molecular medicine, 50, 1-11. doi:10.1038/s12276-018-0147-5. https://pubmed.ncbi.nlm.nih.gov/30213948/
4. Wang, Li, Huang, Guofu, Xiao, Han, Leng, Xiaoling. 2024. A pan-cancer analysis of the association of METRN with prognosis and immune infiltration in human tumors. In Heliyon, 10, e37213. doi:10.1016/j.heliyon.2024.e37213. https://pubmed.ncbi.nlm.nih.gov/39296047/
5. Wang, Xinxin, Cao, Qinchen, Shi, Yonggang, Liu, Zhangsuo, Zhang, Mingzhi. 2021. Identification of low-dose radiation-induced exosomal circ-METRN and miR-4709-3p/GRB14/PDGFRα pathway as a key regulatory mechanism in Glioblastoma progression and radioresistance: Functional validation and clinical theranostic significance. In International journal of biological sciences, 17, 1061-1078. doi:10.7150/ijbs.57168. https://pubmed.ncbi.nlm.nih.gov/33867829/
6. Dai, You-Wu, Ma, Jun-Kai, Jiang, Rui, Chen, Jiong, Lu, Xin-Jiang. . Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization. In Cell reports, 40, 111361. doi:10.1016/j.celrep.2022.111361. https://pubmed.ncbi.nlm.nih.gov/36130501/
7. Sankaranarayanan, Ishwarya, Kume, Moeno, Mohammed, Ayaan, Tavares-Ferreira, Diana, Price, Theodore J. 2024. Persistent changes in nociceptor translatomes govern hyperalgesic priming in mouse models. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.07.606891. https://pubmed.ncbi.nlm.nih.gov/39149295/
8. Garcés, María F, Sanchez, Elizabeth, Cardona, Luisa F, Nogueiras, Rubén, Caminos, Jorge E. 2015. Maternal Serum Meteorin Levels and the Risk of Preeclampsia. In PloS one, 10, e0131013. doi:10.1371/journal.pone.0131013. https://pubmed.ncbi.nlm.nih.gov/26121675/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen