C57BL/6NCya-Hnf4aosem1flox/Cya
Common Name:
Hnf4aos-flox
Product ID:
S-CKO-17771
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hnf4aos-flox
Strain ID
CKOCMP-68314-Hnf4aos-B6N-VA
Gene Name
Product ID
S-CKO-17771
Gene Alias
0610008F07Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Hnf4aosem1flox/Cya mice (Catalog S-CKO-17771) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000141329
NCBI RefSeq
NR_027970
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Hnf4aos, the mouse homolog of human HNF4A-AS1, is a long non-coding RNA. It is located between the P2 and P1 promoters of the HNF4A gene. Hnf4aos is involved in regulating the expression of genes, with potential importance in the development and function of digestive tissues [2].
In a mouse model, liver-specific knockdown of Hnf4aos led to increased serum aspartate and alanine transaminase levels, enlarged and degenerated hepatocytes, as well as increased endoplasmic reticulum stress markers 24 hours after ritonavir (RTV) administration. The aggravated liver injury was due to heightened Cyp3a11 expression, which is the mouse homolog of human CYP3A4. In vitro studies showed that HNF4A-AS1 (the human equivalent) mediated the interaction between heterogeneous nuclear ribonucleoprotein C and HNF4A, with the former promoting HNF4A degradation through the ubiquitination pathway, thus decreasing CYP3A4 expression and alleviating RTV-induced liver injury [1].
In conclusion, Hnf4aos plays a crucial role in regulating CYP3A4 expression, which is associated with RTV-induced hepatotoxicity. The liver-specific knockdown mouse model of Hnf4aos has revealed its important role in this disease-related process, providing insights into the underlying mechanism of RTV-induced liver injury [1].
References：
1. Wang, Xiaofei, Wang, Zijing, Wang, Jingya, Wang, Pei, Zhang, Lirong. 2025. Role of HNF4A-AS1/HNRNPC-mediated HNF4A ubiquitination protection against ritonavir-induced hepatotoxicity. In Molecular pharmacology, 107, 100021. doi:10.1016/j.molpha.2025.100021. https://pubmed.ncbi.nlm.nih.gov/40037142/
2. Guo, Shangdong, Lu, Hong. 2018. Novel mechanisms of regulation of the expression and transcriptional activity of hepatocyte nuclear factor 4α. In Journal of cellular biochemistry, 120, 519-532. doi:10.1002/jcb.27407. https://pubmed.ncbi.nlm.nih.gov/30191603/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen