C57BL/6JCya-Stra8em1flox/Cya
Common Name:
Stra8-flox
Product ID:
S-CKO-17791
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Stra8-flox
Strain ID
CKOCMP-20899-Stra8-B6J-VB
Gene Name
Product ID
S-CKO-17791
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stra8em1flox/Cya mice (Catalog S-CKO-17791) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114999
NCBI RefSeq
NM_009292
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Stra8, or Stimulated By Retinoic Acid Gene 8, is a gene induced by retinoic acid (RA) and plays vital roles in both male and female germ cell development. It is crucial for the transition from mitosis to meiosis in mammalian germ cells, associated with pathways like the cell cycle, apoptosis, and autophagy regulation. Genetic models, especially KO mouse models, have been instrumental in studying its functions [1,2,4,5,6,7].
In female mice, mutation of the RB-binding site of Stra8 delays meiotic entry, meiotic prophase progression, and leads to precocious depletion of the oocyte pool, suggesting Stra8-RB interaction is required for S phase entry and meiotic gene activation [1]. In male mice, Stra8 knockout leads to a muted RA response during spermatogonial development, with more transcripts typical of undifferentiated cells. Although spermatocytes can form, they fail to complete meiosis [2]. Stra8-deficient germ cells in male mice also fail to enter meiosis and show aberrant upregulation of autophagy-lysosome genes, indicating Stra8 suppresses autophagy during spermatogenesis by repressing Nr1d1 expression [7]. Moreover, Stra8 may inhibit apoptosis during mouse spermatogenesis via the AKT signaling pathway as observed in Stra8-knockout male mice [6].
In conclusion, Stra8 is an essential gatekeeper for meiotic initiation in both male and female germ cells. Mouse KO models have revealed its role in various processes related to germ cell development and have implications for understanding infertility-related diseases such as premature ovarian insufficiency, where mutations in Stra8 may contribute to the condition [3].
References:
1. Shimada, Ryuki, Kato, Yuzuru, Takeda, Naoki, Araki, Kimi, Ishiguro, Kei-Ichiro. 2023. STRA8-RB interaction is required for timely entry of meiosis in mouse female germ cells. In Nature communications, 14, 6443. doi:10.1038/s41467-023-42259-6. https://pubmed.ncbi.nlm.nih.gov/37880249/
2. Gewiss, Rachel L, Shelden, Eric A, Griswold, Michael D. 2021. STRA8 induces transcriptional changes in germ cells during spermatogonial development. In Molecular reproduction and development, 88, 128-140. doi:10.1002/mrd.23448. https://pubmed.ncbi.nlm.nih.gov/33400349/
3. Ke, Hanni, Tang, Shuyan, Guo, Ting, Jin, Li, Chen, Zi-Jiang. 2023. Landscape of pathogenic mutations in premature ovarian insufficiency. In Nature medicine, 29, 483-492. doi:10.1038/s41591-022-02194-3. https://pubmed.ncbi.nlm.nih.gov/36732629/
4. Ishiguro, Kei-Ichiro, Matsuura, Kumi, Tani, Naoki, Araki, Kimi, Niwa, Hitoshi. 2020. MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ Cells. In Developmental cell, 52, 429-445.e10. doi:10.1016/j.devcel.2020.01.010. https://pubmed.ncbi.nlm.nih.gov/32032549/
5. Niu, Changmin, Guo, Jiaqian, Shen, Xueyi, Xia, Jing, Zheng, Ying. 2020. Meiotic gatekeeper STRA8 regulates cell cycle by interacting with SETD8 during spermatogenesis. In Journal of cellular and molecular medicine, 24, 4194-4211. doi:10.1111/jcmm.15080. https://pubmed.ncbi.nlm.nih.gov/32090428/
6. Shen, Xueyi, Niu, Changmin, Guo, Jiaqian, Hu, Yanqiu, Zheng, Ying. 2018. Stra8 may inhibit apoptosis during mouse spermatogenesis via the AKT signaling pathway. In International journal of molecular medicine, 42, 2819-2830. doi:10.3892/ijmm.2018.3825. https://pubmed.ncbi.nlm.nih.gov/30106128/
7. Ferder, Ianina C, Fung, Leslie, Ohguchi, Yasuyo, Xavier, Ramnik J, Wang, Ning. 2019. Meiotic gatekeeper STRA8 suppresses autophagy by repressing Nr1d1 expression during spermatogenesis in mice. In PLoS genetics, 15, e1008084. doi:10.1371/journal.pgen.1008084. https://pubmed.ncbi.nlm.nih.gov/31059511/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen