C57BL/6JCya-Kif5bem1flox/Cya
Common Name:
Kif5b-flox
Product ID:
S-CKO-17947
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Kif5b-flox
Strain ID
CKOCMP-16573-Kif5b-B6J-VB
Gene Name
Product ID
S-CKO-17947
Gene Alias
Khc; Khcs; Kns1; Ukhc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kif5bem1flox/Cya mice (Catalog S-CKO-17947) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025083
NCBI RefSeq
NM_008448
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Kif5b, a member of the kinesin family, is an important motor protein involved in microtubule-mediated intracellular transport. It contributes to key cellular processes such as intracellular trafficking, organelle dynamics, and cell division [1]. Ubiquitously expressed, it is distinct from some of its family members like KIF5A and KIF5C which have more specific tissue expressions [1].
In a Kif5b conditional knockout (CKO) mouse model where Kif5b was knocked out in CaMKIIα -Cre-expressing neurons, dendritic spine plasticity was affected. There was heightened turnover and lower stability of dendritic spines in layer 2/3 pyramidal neurons, along with reduced acquisition of spine postsynaptic density protein 95 in the mouse cortex. Also, the preceding transport of RNA-binding protein fragile X mental retardation protein (FMRP) to newly formed spines, which occurs in control mice, was abolished in KIF5B cKO mice. Moreover, the learning-dependent localization of FMRP after fear extinction was disrupted in these cKO mice, indicating Kif5b's role in dendritic targeting of synaptic proteins underlying dendritic spine plasticity [2].
In conclusion, Kif5b is essential for intracellular transport and plays a crucial role in maintaining normal cellular functions. The Kif5b cKO mouse model has revealed its significance in dendritic spine plasticity, which may have implications for understanding neurological processes. This contributes to our knowledge of how disruptions in Kif5b function can lead to various phenotypic changes, highlighting its importance in disease-related research.
References:
1. Flex, Elisabetta, Albadri, Shahad, Radio, Francesca Clementina, Del Bene, Filippo, Tartaglia, Marco. . Dominantly acting KIF5B variants with pleiotropic cellular consequences cause variable clinical phenotypes. In Human molecular genetics, 32, 473-488. doi:10.1093/hmg/ddac213. https://pubmed.ncbi.nlm.nih.gov/36018820/
2. Fok, Albert Hiu Ka, Huang, Yuhua, So, Beth Wing Lam, Lai, Kwok-On, Lai, Cora Sau Wan. 2024. KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo. In Cell reports, 43, 113906. doi:10.1016/j.celrep.2024.113906. https://pubmed.ncbi.nlm.nih.gov/38451812/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen