C57BL/6JCya-Ltbem1flox/Cya
Common Name:
Ltb-flox
Product ID:
S-CKO-18125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ltb-flox
Strain ID
CKOCMP-16994-Ltb-B6J-VB
Gene Name
Product ID
S-CKO-18125
Gene Alias
LTbeta; Tnfc; Tnfsf3; Tnlg1c; p33
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ltbem1flox/Cya mice (Catalog S-CKO-18125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025262
NCBI RefSeq
NM_008518
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Ltb, or leukotriene B4, is a lipid mediator derived from arachidonic acid. It acts as a chemoattractant for inflammatory leukocytes and is a key mediator in a cascade involving complement, lipid, cytokine, and chemokine, which initiates and sustains neutrophilic inflammation in inflammatory arthritis [1,3]. Ltb binds to two receptors, the high-affinity receptor BLT1 and the low-affinity receptor BLT2, and the Ltb/BLT1 axis is involved in leukocyte recruitment to infected sites and pathogen elimination [2].
In Chronic Granulomatous Disease (CGD), a Ltb4/CD11b self-amplifying loop drives pyogranuloma formation. Antagonizing BLT1 blocked neutrophil aggregation in an ex vivo system of pyogranuloma formation using peritoneal cells of zymosan-treated CGD (gp91phox-/-) mice, indicating the importance of Ltb in this hyperinflammatory condition [4]. In Lyme arthritis, macrophages from 5-lipoxygenase (5-LOX)-/-mice, which are defective in Ltb production, showed impaired phagocytosis of Borrelia burgdorferi. Exogenous Ltb addition augmented phagocytic capability, suggesting Ltb's role in macrophage phagocytosis [5].
In conclusion, Ltb is crucial in inflammatory processes, leukocyte recruitment, and phagocytosis. Studies using gene-knockout mouse models, such as in CGD and Lyme arthritis, have revealed its role in specific disease-related biological processes, providing insights into potential therapeutic targets for inflammatory diseases.
References:
1. Miyabe, Yoshishige, Miyabe, Chie, Luster, Andrew D. . LTB4 and BLT1 in inflammatory arthritis. In Seminars in immunology, 33, 52-57. doi:10.1016/j.smim.2017.09.009. https://pubmed.ncbi.nlm.nih.gov/29042029/
2. Saeki, Kazuko, Yokomizo, Takehiko. . Identification, signaling, and functions of LTB4 receptors. In Seminars in immunology, 33, 30-36. doi:10.1016/j.smim.2017.07.010. https://pubmed.ncbi.nlm.nih.gov/29042026/
3. Subramanian, Bhagawat C, Majumdar, Ritankar, Parent, Carole A. . The role of the LTB4-BLT1 axis in chemotactic gradient sensing and directed leukocyte migration. In Seminars in immunology, 33, 16-29. doi:10.1016/j.smim.2017.07.002. https://pubmed.ncbi.nlm.nih.gov/29042024/
4. Haist, Kelsey C, Gibbings, Sophie L, Jacobelli, Jordan, Henson, Peter M, Bratton, Donna L. 2024. A LTB4/CD11b self-amplifying loop drives pyogranuloma formation in chronic granulomatous disease. In iScience, 27, 109589. doi:10.1016/j.isci.2024.109589. https://pubmed.ncbi.nlm.nih.gov/38623335/
5. Zhang, Yan, Olson, Rachel M, Brown, Charles R. 2017. Macrophage LTB4 drives efficient phagocytosis of Borrelia burgdorferi via BLT1 or BLT2. In Journal of lipid research, 58, 494-503. doi:10.1194/jlr.M068882. https://pubmed.ncbi.nlm.nih.gov/28053185/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen