C57BL/6JCya-Slc22a2em1flox/Cya
Common Name:
Slc22a2-flox
Product ID:
S-CKO-18154
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc22a2-flox
Strain ID
CKOCMP-20518-Slc22a2-B6J-VB
Gene Name
Product ID
S-CKO-18154
Gene Alias
Oct2; Orct2; mOCT2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc22a2em1flox/Cya mice (Catalog S-CKO-18154) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046959
NCBI RefSeq
NM_013667
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Slc22a2, encoding the organic cation transporter-2 (OCT2), is a polyspecific transporter in the SLC22 family. It mediates the electrogenic transport of small organic cations with different molecular structures, and is expressed in tissues like the kidney and liver. SLC22 transporters, including Slc22a2, are involved in regulating key metabolic pathways and levels of signaling molecules, and play important roles in interorgan and interorganism small-molecule communication [3,4].
In patients with colorectal cancer receiving oxaliplatin chemotherapy, the rs316019 genotype of Slc22a2 was significantly associated with the severity of leucopenia and neutropenia, and the rs1869641 genotype was associated with the occurrence of thrombocytopenia [1]. In HIV-positive patients treated with dolutegravir, the SLC22A2 CA genotype was independently associated with an abnormal general severity index, anxiety, hostility, and moderate to severe headache [2]. In renal transplantation, SLC22A2 was associated with phenotypes of net tubular creatinine secretion and end-stage renal disease [5]. Also, the rs316019 (p.270A > S) variant of SLC22A2 may reduce the clearance of metformin from circulation, potentially leading to metformin-associated lactic acidosis [6].
In conclusion, Slc22a2 is crucial for the transport of various organic cations, which impacts drug efficacy, toxicity, and metabolic processes. Studies on its genetic variants in different patient populations have revealed its associations with hematological toxicity in colorectal cancer chemotherapy, psychiatric symptoms in HIV patients on dolutegravir, creatinine handling in renal transplantation, and metformin clearance in type 2 diabetes. These findings contribute to understanding the role of Slc22a2 in disease-related drug responses and metabolic regulations.
References:
1. Chen, Jiayin, Wang, Li, Tong, Gangling, Liu, Chaoran, Wang, Lijun. . The SLC22A2 gene is a determinant of hematological toxicity of oxaliplatin in patients with colorectal cancer. In International journal of clinical pharmacology and therapeutics, 61, 1-7. doi:10.5414/CP204156. https://pubmed.ncbi.nlm.nih.gov/36331012/
2. Borghetti, A, Calcagno, A, Lombardi, F, Bonora, S, Di Giambenedetto, S. . SLC22A2 variants and dolutegravir levels correlate with psychiatric symptoms in persons with HIV. In The Journal of antimicrobial chemotherapy, 74, 1035-1043. doi:10.1093/jac/dky508. https://pubmed.ncbi.nlm.nih.gov/30561642/
3. Pearce, Brendon, Jacobs, Clifford, Hoosain, Nisreen, Benjeddou, Mongi. . SLC22A2 - mapping genomic variations within South African indigenous and admixed populations. In Drug metabolism and personalized therapy, 31, 213-220. doi:10.1515/dmpt-2016-0022. https://pubmed.ncbi.nlm.nih.gov/27828777/
4. Nigam, Sanjay K. . The SLC22 Transporter Family: A Paradigm for the Impact of Drug Transporters on Metabolic Pathways, Signaling, and Disease. In Annual review of pharmacology and toxicology, 58, 663-687. doi:10.1146/annurev-pharmtox-010617-052713. https://pubmed.ncbi.nlm.nih.gov/29309257/
5. Reznichenko, Anna, Sinkeler, Steef J, Snieder, Harold, Seelen, Marc, Navis, Gerjan. 2013. SLC22A2 is associated with tubular creatinine secretion and bias of estimated GFR in renal transplantation. In Physiological genomics, 45, 201-9. doi:10.1152/physiolgenomics.00087.2012. https://pubmed.ncbi.nlm.nih.gov/23341218/
6. Sajib, Abu Ashfaqur, Islam, Tasmia, Paul, Nilanjana, Yeasmin, Sabina. 2018. Interaction of rs316019 variants of SLC22A2 with metformin and other drugs- an in silico analysis. In Journal, genetic engineering & biotechnology, 16, 769-775. doi:10.1016/j.jgeb.2018.01.003. https://pubmed.ncbi.nlm.nih.gov/30733798/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen