C57BL/6JCya-Mobpem1flox/Cya
Common Name:
Mobp-flox
Product ID:
S-CKO-18182
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mobp-flox
Strain ID
CKOCMP-17433-Mobp-B6J-VA
Gene Name
Product ID
S-CKO-18182
Gene Alias
MOBP155; MOBP170; MOBP69; MOBP73; MOBP81
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mobpem1flox/Cya mice (Catalog S-CKO-18182) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000068698
NCBI RefSeq
NM_001039365
Target Region
Exon 3~4
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Mobp, short for myelin-associated oligodendrocyte basic protein, is a relatively abundant CNS-specific myelin protein. It plays a crucial role in stabilizing the myelin sheath in the central nervous system (CNS), and its synthesis is regulated by Fyn kinase, which also affects the morphological differentiation of oligodendrocytes [3,5]. Myelination is essential for rapid saltatory conduction of action potentials within the CNS and maintaining neuronal health, and Mobp is thus important for normal CNS function.
In multiple system atrophy (MSA), decreased MOBP mRNA levels are significantly correlated with increased DNA methylation. MOBP is also mislocalized into the glial cytoplasmic inclusions (GCIs) in MSA, where it interacts with α-synuclein, emphasizing its relevance to the pathogenesis of MSA [2]. In multiple sclerosis (MS), T-cell autoimmunity against MOBP can be detected, and MOBP-reactive T-cells can be pathogenic in some mouse strains, suggesting it is a relevant primary target autoantigen in MS [3]. A study on sporadic frontotemporal dementia (sFTD) found a candidate locus on chromosome 3 in the intergenic region between RPSA and MOBP, contributing to increased risk for sFTD through effects on expression and/or splicing in brain cortex [1]. However, a case-control study in a Greek population found no association between MOBP rs616147 polymorphism and amyotrophic lateral sclerosis (ALS) [4].
In conclusion, Mobp is vital for myelin sheath stability and oligodendrocyte morphological differentiation in the CNS. Its role has been revealed in diseases such as MSA, MS, and potentially sFTD through various research models. Although no link was found between a specific polymorphism and ALS in one study, further research could potentially clarify its role in other neurodegenerative diseases and complex genetic disorders.
References:
1. Manzoni, Claudia, Kia, Demis A, Ferrari, Raffaele, Hardy, John, Escott-Price, Valentina. 2024. Genome-wide analyses reveal a potential role for the MAPT, MOBP, and APOE loci in sporadic frontotemporal dementia. In American journal of human genetics, 111, 1316-1329. doi:10.1016/j.ajhg.2024.05.017. https://pubmed.ncbi.nlm.nih.gov/38889728/
2. Bettencourt, Conceição, Miki, Yasuo, Piras, Ignazio S, Huentelman, Matt J, Holton, Janice L. 2021. MOBP and HIP1 in multiple system atrophy: New α-synuclein partners in glial cytoplasmic inclusions implicated in the disease pathogenesis. In Neuropathology and applied neurobiology, 47, 640-652. doi:10.1111/nan.12688. https://pubmed.ncbi.nlm.nih.gov/33368549/
3. Kaushansky, Nathali, Eisenstein, Miriam, Zilkha-Falb, Rina, Ben-Nun, Avraham. 2009. The myelin-associated oligodendrocytic basic protein (MOBP) as a relevant primary target autoantigen in multiple sclerosis. In Autoimmunity reviews, 9, 233-6. doi:10.1016/j.autrev.2009.08.002. https://pubmed.ncbi.nlm.nih.gov/19683076/
4. Liampas, Ioannis, Siokas, Vasileios, Aloizou, Athina-Maria, Hadjigeorgiou, Georgios M, Dardiotis, Efthimios. 2021. MOBP rs616147 Polymorphism and Risk of Amyotrophic Lateral Sclerosis in a Greek Population: A Case-Control Study. In Medicina (Kaunas, Lithuania), 57, . doi:10.3390/medicina57121337. https://pubmed.ncbi.nlm.nih.gov/34946282/
5. Schäfer, Isabelle, Müller, Christina, Luhmann, Heiko J, White, Robin. 2016. MOBP levels are regulated by Fyn kinase and affect the morphological differentiation of oligodendrocytes. In Journal of cell science, 129, 930-42. doi:10.1242/jcs.172148. https://pubmed.ncbi.nlm.nih.gov/26801084/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen