C57BL/6JCya-Sema6bem1flox/Cya
Common Name:
Sema6b-flox
Product ID:
S-CKO-18277
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sema6b-flox
Strain ID
CKOCMP-20359-Sema6b-B6J-VB
Gene Name
Product ID
S-CKO-18277
Gene Alias
Sema; Seman; VIb; semaZ
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sema6bem1flox/Cya mice (Catalog S-CKO-18277) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001256
NCBI RefSeq
NM_001130456
Target Region
Exon 4~7
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Sema6b, a member of the semaphorin axon-guidance family, is involved in multiple biological processes. It plays important roles in axon guidance, neuronal development such as spine formation and maturation, and may be associated with immune-related pathways and inflammation [2].
In the context of hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF), Sema6b has been found to be a potential diagnostic biomarker. Knockout of Sema6b in mice rescued liver failure by improving liver functions, reducing inflammatory responses, and decreasing hepatocyte apoptosis. Overexpression of Sema6b in macrophages activated systemic inflammatory responses, while in hepatocytes it inhibited proliferation and induced apoptosis [1].
In conclusion, Sema6b is crucial for neuronal development and axon guidance. In disease, especially HBV-ACLF, Sema6b plays a role in promoting liver failure through macrophage-mediated inflammation and hepatocyte apoptosis. The use of Sema6b knockout mouse models has been instrumental in revealing its role in liver-related disease conditions, highlighting its potential as a therapeutic target for HBV-ACLF.
References：
1. Yang, Hui, Cai, Qun, Xin, Jiaojiao, Shi, Dongyan, Li, Jun. 2024. SEMA6B induces macrophage-mediated inflammation and hepatocyte apoptosis in hepatitis B virus-related acute-on-chronic liver failure. In Theranostics, 14, 5200-5218. doi:10.7150/thno.97007. https://pubmed.ncbi.nlm.nih.gov/39267780/
2. Cordovado, Amélie, Schaettin, Martina, Jeanne, Médéric, Toutain, Annick, Vuillaume, Marie-Laure. . SEMA6B variants cause intellectual disability and alter dendritic spine density and axon guidance. In Human molecular genetics, 31, 3325-3340. doi:10.1093/hmg/ddac114. https://pubmed.ncbi.nlm.nih.gov/35604360/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen