C57BL/6JCya-Chatem1flox/Cya
Common Name:
Chat-flox
Product ID:
S-CKO-18281
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Chat-flox
Strain ID
CKOCMP-12647-Chat-B6J-VB
Gene Name
Product ID
S-CKO-18281
Gene Alias
B230380D24Rik; CHOACTase
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chatem1flox/Cya mice (Catalog S-CKO-18281) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070125
NCBI RefSeq
NM_009891
Target Region
Exon 4~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Chat, short for choline acetyltransferase, is a synthesizing enzyme for acetylcholine (ACh), a neurotransmitter crucial for nerve impulse transmission in both the central and peripheral nervous systems [1]. It catalyzes the transfer of an acetyl group from coenzyme acetyl-CoA to choline to yield ACh. ChAT-expressing neurons play significant roles in various neural functions, and the creation of transgenic mouse models like the ChAT-eGFP transgenic mice has provided advantages for studying cholinergic neurons [1].
In the ChAT-eGFP transgenic mouse brain study, while there was a complete overlap between the transgenic expression of eGFP and the immunohistochemical staining in the cholinergic basal forebrain, in other areas such as the cortex, hippocampus, and brainstem, differences in the distribution of ChAT-eGFP expressing neurons and ChAT immunoreactive neurons were observed. This indicates that caution should be exercised when using this transgenic mouse line for studying cholinergic neurons in specific brain regions [1].
In conclusion, Chat is essential for the synthesis of acetylcholine, a key neurotransmitter. The use of transgenic mouse models, like the ChAT-eGFP transgenic mice, has revealed differences in the distribution of ChAT-expressing neurons in different brain regions. This contributes to our understanding of the role of cholinergic neurons in neural functions and could potentially aid in research related to neurological diseases where cholinergic function may be disrupted.
References:
1. Gamage, Rashmi, Zaborszky, Laszlo, Münch, Gerald, Gyengesi, Erika. 2023. Evaluation of eGFP expression in the ChAT-eGFP transgenic mouse brain. In BMC neuroscience, 24, 4. doi:10.1186/s12868-023-00773-9. https://pubmed.ncbi.nlm.nih.gov/36650430/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen