C57BL/6JCya-Nf2em1flox/Cya
Common Name:
Nf2-flox
Product ID:
S-CKO-18499
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nf2-flox
Strain ID
CKOCMP-18016-Nf2-B6J-VB
Gene Name
Product ID
S-CKO-18499
Gene Alias
merlin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nf2em1flox/Cya mice (Catalog S-CKO-18499) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109910
NCBI RefSeq
NM_010898
Target Region
Exon 6
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
NF2, also known as Neurofibromatosis type 2 gene, encodes the tumor suppressor protein Merlin, a member of the ezrin, radixin, and moesin (ERM) family [2,3,5,6]. Merlin regulates cytoskeleton and cell signaling, and is involved in crucial pathways such as the Hippo tumor-suppressive and mTOR pro-oncogenic signaling cascades [1]. The NF2 gene is of great biological importance as its inactivation is linked to tumorigenesis [1,2,3,5,6,7,8].
NF2 inactivation occurs in 30%-40% of mesotheliomas, potentially a late event conferring a more aggressive phenotype [1]. NF2 mutations are specifically associated with the development of schwannomas, meningiomas, and ependymomas [2]. Meningioma cells with NF2 inactivation are susceptible to Erastin-induced ferroptosis, and maintaining NF2 inhibits ferroptosis-related lipid peroxidation and cell death [4]. NF2-related schwannomatosis is characterized by the growth of benign nervous system tumors due to NF2 gene mutations and Merlin dysfunction [5]. In NF2 patients, mutations in the NF2 gene lead to loss of function of Merlin, strongly associated with the development of vestibular schwannomas and meningiomas [7]. NF2 gene mutations are implicated in over 11 different cancers, with 8 of them showing evidence of Hippo signaling cascade involvement [8].
In conclusion, NF2, through its product Merlin, plays a vital role in regulating cell-signaling pathways. Model-based research, especially studies on NF2 inactivation, has revealed its significance in various tumor-related diseases such as mesothelioma, schwannomas, meningiomas, and ependymomas. Understanding NF2 is crucial for elucidating tumorigenesis mechanisms and developing potential therapeutic strategies.
References:
1. Sekido, Yoshitaka, Sato, Tatsuhiro. 2023. NF2 alteration in mesothelioma. In Frontiers in toxicology, 5, 1161995. doi:10.3389/ftox.2023.1161995. https://pubmed.ncbi.nlm.nih.gov/37180489/
2. Vlashi, Rexhina, Sun, Fuju, Zheng, Chenggong, Liu, Jie, Chen, Guiqian. . The molecular biology of NF2/Merlin on tumorigenesis and development. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, e23809. doi:10.1096/fj.202400019RR. https://pubmed.ncbi.nlm.nih.gov/38967126/
3. Xu, Duo, Yin, Shiyuan, Shu, Yongqian. 2024. NF2: An underestimated player in cancer metabolic reprogramming and tumor immunity. In NPJ precision oncology, 8, 133. doi:10.1038/s41698-024-00627-5. https://pubmed.ncbi.nlm.nih.gov/38879686/
4. Bao, Zhongyuan, Hua, Lingyang, Ye, Yangfan, Gong, Ye, Ji, Jing. . MEF2C silencing downregulates NF2 and E-cadherin and enhances Erastin-induced ferroptosis in meningioma. In Neuro-oncology, 23, 2014-2027. doi:10.1093/neuonc/noab114. https://pubmed.ncbi.nlm.nih.gov/33984142/
5. Kim, Bae-Hoon, Chung, Yeon-Ho, Woo, Tae-Gyun, Kim, Minju, Park, Bum-Joon. 2024. NF2-Related Schwannomatosis (NF2): Molecular Insights and Therapeutic Avenues. In International journal of molecular sciences, 25, . doi:10.3390/ijms25126558. https://pubmed.ncbi.nlm.nih.gov/38928264/
6. Xiao, Guang-Hui, Chernoff, Jonathan, Testa, Joseph R. . NF2: the wizardry of merlin. In Genes, chromosomes & cancer, 38, 389-99. doi:. https://pubmed.ncbi.nlm.nih.gov/14566860/
7. Bachir, Suha, Shah, Sanjit, Shapiro, Scott, Schorry, Elizabeth, Sengupta, Soma. 2021. Neurofibromatosis Type 2 (NF2) and the Implications for Vestibular Schwannoma and Meningioma Pathogenesis. In International journal of molecular sciences, 22, . doi:10.3390/ijms22020690. https://pubmed.ncbi.nlm.nih.gov/33445724/
8. Nouri, Shervin Hosseingholi, Nitturi, Vijay, Ledbetter, Elizabeth, Klisch, Tiemo J, Patel, Akash J. 2024. Role of NF2 Mutation in the Development of Eleven Different Cancers. In Cancers, 17, . doi:10.3390/cancers17010064. https://pubmed.ncbi.nlm.nih.gov/39796693/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen