C57BL/6JCya-Zkscan1em1flox/Cya
Common Name:
Zkscan1-flox
Product ID:
S-CKO-18616
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zkscan1-flox
Strain ID
CKOCMP-74570-Zkscan1-B6J-VB
Gene Name
Product ID
S-CKO-18616
Gene Alias
5930429A01Rik; 9130423L19Rik; 9230118B16Rik; KOX18; PHZ-37; ZNF139
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zkscan1em1flox/Cya mice (Catalog S-CKO-18616) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019660
NCBI RefSeq
NM_133906
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
ZKSCAN1, a zinc finger family gene, is expressed in both linear and circular (circZKSCAN1) forms of RNA [5]. It has been linked to multiple biological processes and disease conditions. ZKSCAN1 has recently been identified as a regulator of histone mRNA polyadenylation-mediated inflammation, acting as a novel RNA binding protein [6]. It has also been found to interact with ATXN1, with altered expression of its target genes observed in SCA1 mice and patient-derived iNeurons, suggesting its potential contribution to spinocerebellar ataxia type 1 pathogenesis [7].
In cancer research, studies on circZKSCAN1, a circular RNA derived from ZKSCAN1, have shown diverse functions. In hepatocellular carcinoma (HCC), circZKSCAN1-encoded peptide circZKSaa suppresses HCC via degradation of mTOR, inhibiting the PI3K/AKT/mTOR pathway, and could be a biomarker and therapeutic target [1]. Additionally, both ZKSCAN1 mRNA and circZKSCAN1 inhibit HCC cell growth, migration, and invasion through different signaling pathways, with ZKSCAN1 mRNA mainly regulating cellular metabolism and circZKSCAN1 mediating cancer-related signaling pathways [5]. In lung adenocarcinoma, circ-ZKSCAN1 promotes tumorigenesis and cisplatin resistance by regulating the miR-185-5p/TAGLN2 axis [2]. In non-small cell lung cancer, circ-ZKSCAN1 promotes progression by acting as a sponge of miR-330-5p to increase FAM83A expression and inactivate the MAPK signaling pathway [3]. Conversely, in bladder cancer, circZKSCAN1 inhibits cancer progression through the miR-1178-3p/p21 axis and can serve as a prognostic factor of recurrence [4].
In conclusion, ZKSCAN1 and its circular RNA counterpart play significant roles in multiple biological processes, especially in cancer and neurodegenerative diseases. Research on ZKSCAN1, particularly through studies on its circular RNA forms in cancer models, has provided insights into disease mechanisms, highlighting its potential as a biomarker and therapeutic target in cancer treatment, and also suggesting its importance in understanding the pathogenesis of spinocerebellar ataxia type 1.
References：
1. Song, Runjie, Ma, Shuoqian, Xu, Jiajia, Li, Guangming, Li, Xiangdong. 2023. A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR. In Molecular cancer, 22, 16. doi:10.1186/s12943-023-01719-9. https://pubmed.ncbi.nlm.nih.gov/36691031/
2. Yu, Nanbin, Gong, Huaijun, Chen, Wei, Peng, Weixing. 2023. CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR-185-5p/TAGLN2 axis. In Thoracic cancer, 14, 1467-1476. doi:10.1111/1759-7714.14889. https://pubmed.ncbi.nlm.nih.gov/37105934/
3. Wang, Yuanyong, Xu, Rongjian, Zhang, Dongyang, Wang, Jianxun, Jiao, Wenjie. . Circ-ZKSCAN1 regulates FAM83A expression and inactivates MAPK signaling by targeting miR-330-5p to promote non-small cell lung cancer progression. In Translational lung cancer research, 8, 862-875. doi:10.21037/tlcr.2019.11.04. https://pubmed.ncbi.nlm.nih.gov/32010565/
4. Bi, Junming, Liu, Hongwei, Dong, Wei, Huang, Jian, Lin, Tianxin. 2019. Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence. In Molecular cancer, 18, 133. doi:10.1186/s12943-019-1060-9. https://pubmed.ncbi.nlm.nih.gov/31481066/
5. Yao, Zhicheng, Luo, Jingyan, Hu, Kunpeng, Liu, Bo, Yang, Yang. 2017. ZKSCAN1 gene and its related circular RNA (circZKSCAN1) both inhibit hepatocellular carcinoma cell growth, migration, and invasion but through different signaling pathways. In Molecular oncology, 11, 422-437. doi:10.1002/1878-0261.12045. https://pubmed.ncbi.nlm.nih.gov/28211215/
6. Guo, Mengbiao, Zhu, Jianxi, Hu, Zhijie, Songyang, Zhou, Xiong, Yuanyan. . Histone mRNA polyadenylation-mediated inflammation underlies various virus infections and cancers. In Journal of medical virology, 95, e28826. doi:10.1002/jmv.28826. https://pubmed.ncbi.nlm.nih.gov/37254821/
7. Coffin, Stephanie L, Durham, Mark A, Nitschke, Larissa, Orr, Harry T, Zoghbi, Huda Y. 2022. Disruption of the ATXN1-CIC complex reveals the role of additional nuclear ATXN1 interactors in spinocerebellar ataxia type 1. In Neuron, 111, 481-492.e8. doi:10.1016/j.neuron.2022.11.016. https://pubmed.ncbi.nlm.nih.gov/36577402/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen