C57BL/6JCya-Camk1dem1flox/Cya
Common Name:
Camk1d-flox
Product ID:
S-CKO-18640
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Camk1d-flox
Strain ID
CKOCMP-227541-Camk1d-B6J-VB
Gene Name
Product ID
S-CKO-18640
Gene Alias
A630059D12Rik; CKLiK; CaMKIdelta; E030025C11Rik; mCKLiK
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Camk1dem1flox/Cya mice (Catalog S-CKO-18640) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044009
NCBI RefSeq
NM_177343
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Camk1d, also known as calcium/calmodulin-dependent protein kinase ID, is involved in multiple intracellular signaling pathways. It has been associated with the PI3K/AKT/mTOR pathway and is important in various biological processes. It is widely expressed in many tissues and plays a role in cell growth, making it relevant in understanding disease mechanisms [2].
Global Camk1d-knockout male mice are resistant to ghrelin, gain less body weight and are protected against high-fat-diet-induced obesity. Deletion of Camk1d in AgRP/NPY neurons recapitulates these phenotypes. In response to ghrelin, lack of CaMK1D attenuates phosphorylation of CREB and CREB-dependent expression of orexigenic neuropeptides in fibre projections to the PVN, indicating its role in ghrelin-mediated food intake [1]. In glioma, knockdown of CamK1D promotes cell proliferation, invasion and migration, while overexpression has the opposite effects, suggesting its tumor-inhibitory role through the PI3K/AKT/mTOR pathway [2].
In conclusion, Camk1d is crucial in regulating ghrelin-mediated food intake and body weight, as shown by knockout mouse models. In addition, it plays a role in tumor-related processes such as glioma cell function. These findings from Camk1d KO models contribute to understanding the mechanisms of obesity-related diseases and glioma, providing potential targets for treatment.
References:
1. Vivot, Karl, Meszaros, Gergö, Pangou, Evanthia, Nogueiras, Ruben, Ricci, Romeo. 2023. CaMK1D signalling in AgRP neurons promotes ghrelin-mediated food intake. In Nature metabolism, 5, 1045-1058. doi:10.1038/s42255-023-00814-x. https://pubmed.ncbi.nlm.nih.gov/37277610/
2. Jin, Qianxu, Zhao, Jiahui, Zhao, Zijun, Liu, Liping, Zhao, Zongmao. 2022. CAMK1D Inhibits Glioma Through the PI3K/AKT/mTOR Signaling Pathway. In Frontiers in oncology, 12, 845036. doi:10.3389/fonc.2022.845036. https://pubmed.ncbi.nlm.nih.gov/35494053/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen