C57BL/6JCya-Mmp10em1flox/Cya
Common Name:
Mmp10-flox
Product ID:
S-CKO-18822
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mmp10-flox
Strain ID
CKOCMP-17384-Mmp10-B6J-VB
Gene Name
Product ID
S-CKO-18822
Gene Alias
MMP-10; SL-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mmp10em1flox/Cya mice (Catalog S-CKO-18822) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034488
NCBI RefSeq
NM_019471
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Mmp10, also known as Stromelysin-2, is a matrix metalloproteinase. It is involved in multiple biological processes, such as the immune response, and is associated with pathways like PPARγ-STAT3, Wnt/β-catenin, and NF-κB signaling. Genetic models, like KO mouse models, have been crucial in understanding its functions [1,2,6,7,8].
In a NASH model, Mmp10-KO and Mmp10-OE mice studies showed that Mmp10 is downregulated in NASH. Overexpression of Mmp10 alleviates hepatic steatosis and inflammation by regulating macrophage M2 polarization through the PPARγ-STAT3 pathway [1]. In a TLR7-mediated tolerance model, Mmp10-/-mice failed to develop immune tolerance to TLR7 ligand, indicating Mmp10 is essential for this process [2]. In tongue cancer, MMP10 knockdown in mice suppressed nodal metastases [3]. For ischemic stroke, rs17860949 polymorphism in Mmp10 was associated with a reduced risk in the Chinese Han population [4]. In a diabetic murine model of ischemic stroke, MMP10 treatment was more effective than tPA in reducing infarct size and neurodegeneration [5]. In tracheal fibrosis, SOX9 promotes ECM deposition by increasing MMP10 expression, and SOX9 knockdown in vivo ameliorated tracheal fibrosis [6]. In colitis and CAC models, miR-148/152 family deficiency in mice elevated MMP10, disrupting the intestinal barrier and activating NF-κB signaling [7]. In a Pseudomonas aeruginosa infection model, Mmp10-/-mice had increased morbidity, and adoptive transfer of wild-type macrophages normalized the condition, showing MMP10's role in moderating the pro-inflammatory response of macrophages [8]. In NSCLC, MMP10 knockdown in A549 cells initiated radiosensitization, as MMP10 is involved in DNA damage repair post-ionizing radiation [9]. In oral squamous cell carcinoma, MMP10 expression increased with nodal level, suggesting its role as a prognostic indicator [10].
In conclusion, Mmp10 plays diverse and significant roles in multiple disease conditions including NASH, immune tolerance, cancer metastasis, ischemic stroke, tracheal fibrosis, colitis, and NSCLC. The use of KO mouse models has been instrumental in revealing these functions, providing insights into potential therapeutic targets for these diseases.
References:
1. Chang, Ling, Gao, Junda, Yu, Yeping, Zhou, Tao, Xu, Qihua. 2023. MMP10 alleviates non-alcoholic steatohepatitis by regulating macrophage M2 polarization. In International immunopharmacology, 124, 111045. doi:10.1016/j.intimp.2023.111045. https://pubmed.ncbi.nlm.nih.gov/37844469/
2. Rohani, Maryam G, Dimitrova, Elizabeth, Beppu, Andrew, Jefferies, Caroline A, Parks, William C. 2018. Macrophage MMP10 Regulates TLR7-Mediated Tolerance. In Frontiers in immunology, 9, 2817. doi:10.3389/fimmu.2018.02817. https://pubmed.ncbi.nlm.nih.gov/30564235/
3. Dharavath, Bhasker, Butle, Ashwin, Pal, Ankita, Nair, Sudhir, Dutt, Amit. 2023. Role of miR-944/MMP10/AXL- axis in lymph node metastasis in tongue cancer. In Communications biology, 6, 57. doi:10.1038/s42003-023-04437-6. https://pubmed.ncbi.nlm.nih.gov/36650344/
4. Zhao, Yong, Zhang, Qi, Zhang, Xiaobo, Shi, Wenzhen, Tian, Ye. 2022. The roles of MMP8/MMP10 polymorphisms in ischemic stroke susceptibility. In Brain and behavior, 12, e2797. doi:10.1002/brb3.2797. https://pubmed.ncbi.nlm.nih.gov/36282475/
5. Navarro-Oviedo, Manuel, Roncal, Carmen, Salicio, Agustina, Muñoz, Roberto, Orbe, Josune. 2018. MMP10 Promotes Efficient Thrombolysis After Ischemic Stroke in Mice with Induced Diabetes. In Translational stroke research, 10, 389-401. doi:10.1007/s12975-018-0652-9. https://pubmed.ncbi.nlm.nih.gov/30051168/
6. Gu, Lei, Li, Anmao, He, Chunyan, Li, Yishi, Guo, Shuliang. 2023. Profibrotic role of the SOX9-MMP10-ECM biosynthesis axis in the tracheal fibrosis after injury and repair. In Genes & diseases, 11, 101040. doi:10.1016/j.gendis.2023.06.012. https://pubmed.ncbi.nlm.nih.gov/38993791/
7. Tang, Kaiwen, Wu, Zhonghua, Sun, Mingwei, Song, Yongxi, Wang, Zhenning. 2022. Elevated MMP10/13 mediated barrier disruption and NF-κB activation aggravate colitis and colon tumorigenesis in both individual or full miR-148/152 family knockout mice. In Cancer letters, 529, 53-69. doi:10.1016/j.canlet.2021.12.033. https://pubmed.ncbi.nlm.nih.gov/34979166/
8. McMahan, Ryan S, Birkland, Timothy P, Smigiel, Kate S, Gharib, Sina A, Parks, William C. 2016. Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation. In Journal of immunology (Baltimore, Md. : 1950), 197, 899-909. doi:10.4049/jimmunol.1600502. https://pubmed.ncbi.nlm.nih.gov/27316687/
9. Bi, Yawei, Cao, Kun, Wang, Yuan, Lei, Xiao, Chen, Yuanyuan. 2023. Radiosensitivity in Non-Small-Cell Lung Cancer by MMP10 through the DNA Damage Repair Pathway. In Journal of oncology, 2023, 5636852. doi:10.1155/2023/5636852. https://pubmed.ncbi.nlm.nih.gov/36908704/
10. Savita, J K, Varsha, V K, Girish, H C. 2023. Role of MMP1 and MMP10 in local invasion and distant metastasis in different levels of oral squamous cell carcinoma - A immunohistochemical comparative study. In Journal of oral and maxillofacial pathology : JOMFP, 27, 315-322. doi:10.4103/jomfp.jomfp_438_22. https://pubmed.ncbi.nlm.nih.gov/37854907/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen