C57BL/6JCya-Ulk2em1flox/Cya
Common Name:
Ulk2-flox
Product ID:
S-CKO-18985
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Ulk2-flox
Strain ID
CKOCMP-29869-Ulk2-B6J-VB
Gene Name
Product ID
S-CKO-18985
Gene Alias
A830085I22Rik; Unc51.2; mKIAA0623
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ulk2em1flox/Cya mice (Catalog S-CKO-18985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004920
NCBI RefSeq
NM_013881
Target Region
Exon 4~5
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Ulk2, short for Unc-51 like autophagy activating kinase 2, is a homolog of the yeast autophagy related protein 1. It plays a crucial role in autophagy, an important cellular process, and is associated with multiple pathways such as insulin signaling, interferon response, and protein degradation [2,3,5]. Genetic models, like gene knockout mouse models, are valuable for studying its functions.
In skeletal muscle, ULK2 deficiency leads to accumulations of insoluble ubiquitinated protein aggregates, impaired muscle force, myofiber atrophy, and degeneration, indicating its unique role in basal selective protein degradation [4].
In ovarian cancer, ULK2 is significantly downregulated, and its low expression is correlated with decreased overall survival. Overexpression of ULK2 suppresses tumor cell proliferation, migration, and invasion by upregulating IGFBP3, suggesting it acts as a tumor suppressor [1].
In gastric cancer, methylation silencing of ULK2 via epithelial-mesenchymal transition can induce cell migration and EMT, causing transformation to poorly differentiated cancers [6].
In glioma patients, low ULK2 expression is associated with poor overall survival, highlighting its potential as a prognostic biomarker [7].
In conclusion, Ulk2 is essential for maintaining protein homeostasis in skeletal muscle and has a significant impact on cancer development and prognosis, as revealed through model-based research. The study of Ulk2 using gene knockout mouse models provides valuable insights into its functions in specific biological processes and disease conditions, especially in cancer-related areas.
References：
1. Chen, Xiaoxi, Shao, Changxiang, Liu, Jing, Xu, Han, Zhu, Weipei. 2024. ULK2 suppresses ovarian cancer cell migration and invasion by elevating IGFBP3. In PeerJ, 12, e17628. doi:10.7717/peerj.17628. https://pubmed.ncbi.nlm.nih.gov/38952983/
2. Xue, Qian, Kang, Rui, Klionsky, Daniel J, Liu, Jinbao, Chen, Xin. 2023. Copper metabolism in cell death and autophagy. In Autophagy, 19, 2175-2195. doi:10.1080/15548627.2023.2200554. https://pubmed.ncbi.nlm.nih.gov/37055935/
3. Ge, Eva J, Bush, Ashley I, Casini, Angela, Brady, Donita C, Chang, Christopher J. 2021. Connecting copper and cancer: from transition metal signalling to metalloplasia. In Nature reviews. Cancer, 22, 102-113. doi:10.1038/s41568-021-00417-2. https://pubmed.ncbi.nlm.nih.gov/34764459/
4. Fuqua, Jordan D, Mere, Caleb P, Kronemberger, Ana, Adams, Christopher M, Lira, Vitor A. 2019. ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33, 11735-11745. doi:10.1096/fj.201900766R. https://pubmed.ncbi.nlm.nih.gov/31361156/
5. Linares, Juan F, Zhang, Xiao, Martinez-Ordoñez, Anxo, Diaz-Meco, Maria T, Moscat, Jorge. 2021. PKCλ/ι inhibition activates an ULK2-mediated interferon response to repress tumorigenesis. In Molecular cell, 81, 4509-4526.e10. doi:10.1016/j.molcel.2021.08.039. https://pubmed.ncbi.nlm.nih.gov/34560002/
6. Motoo, Iori, Nanjo, Sohachi, Ando, Takayuki, Ushijima, Toshikazu, Yasuda, Ichiro. 2021. Methylation silencing of ULK2 via epithelial-mesenchymal transition causes transformation to poorly differentiated gastric cancers. In Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 25, 325-335. doi:10.1007/s10120-021-01250-0. https://pubmed.ncbi.nlm.nih.gov/34554345/
7. Amin, Wajiha, Enam, Syed Ather, Sufiyan, Sufiyan, Abidi, Syed Hani, Mughal, Nouman. 2024. Autophagy-associated biomarkers ULK2, UVRAG, and miRNAs miR-21, miR-126, and miR-374: Prognostic significance in glioma patients. In PloS one, 19, e0311308. doi:10.1371/journal.pone.0311308. https://pubmed.ncbi.nlm.nih.gov/39348350/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen