C57BL/6JCya-Hnrnpul1em1flox/Cya
Common Name:
Hnrnpul1-flox
Product ID:
S-CKO-19245
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hnrnpul1-flox
Strain ID
CKOCMP-232989-Hnrnpul1-B6J-VB
Gene Name
Product ID
S-CKO-19245
Gene Alias
E130317O14Rik; E1B-AP5; E1BAP5; Hnrnpul; Hnrpul1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hnrnpul1em1flox/Cya mice (Catalog S-CKO-19245) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000206832
NCBI RefSeq
NM_144922
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Hnrnpul1, short for heterogeneous nuclear ribonucleoprotein U-like 1, belongs to the family of RNA-binding proteins. It is involved in nucleic acid binding, transcription, and splicing, either by directly binding to DNA and RNA or interacting with other proteins in the spliceosome. These functions suggest its importance in various biological processes and its potential role in human development due to its cross-species conservation [1].
In zebrafish, Hnrnpul1 mutants exhibit reduced body and fin growth, missing bones, craniofacial tendon growth defects, and adult-onset caudal scoliosis, demonstrating its role in skeletal and limb development [1]. In esophageal squamous cell carcinoma, reduced Hnrnpul1 expression enhances cisplatin sensitivity, suggesting its potential as a chemotherapy target [2]. In cervical cancer, NAT10-mediated RNA acetylation enhances Hnrnpul1 mRNA stability, promoting cancer progression, indicating the NAT10-ac4C-Hnrnpul1 axis might be a potential therapeutic target [3]. In ovarian cancer, AKAP8 regulates hnRNPUL1 transcription, promoting cancer progression and antagonizing PARP inhibitor sensitivity [4]. In bladder cancer, LINC00461 promotes epithelial-mesenchymal transition through the miR-518b/Hnrnpul1 axis, suggesting LINC00461 as a potential biomarker and therapeutic target [5]. Also, arginine methylation of hnRNPUL1 by PRMT1 is crucial for its interaction with NBS1 and recruitment to DNA damage sites, highlighting its role in DNA damage response [6].
In summary, Hnrnpul1 plays essential roles in development, such as skeletal and limb formation, and in disease processes including multiple types of cancer and DNA damage response. Research on Hnrnpul1 using models like zebrafish and through functional studies in cancer cells has provided valuable insights into its biological functions and potential as a therapeutic target in various disease areas.
References:
1. Blackwell, Danielle L, Fraser, Sherri D, Caluseriu, Oana, Bernier, François P, Childs, Sarah J. . Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo. In G3 (Bethesda, Md.), 12, . doi:10.1093/g3journal/jkac067. https://pubmed.ncbi.nlm.nih.gov/35325113/
2. Li, Juan, Sang, Meixiang, Zheng, Yang, Li, Weijing, Shan, Baoen. 2021. HNRNPUL1 inhibits cisplatin sensitivity of esophageal squamous cell carcinoma through regulating the formation of circMAN1A2. In Experimental cell research, 409, 112891. doi:10.1016/j.yexcr.2021.112891. https://pubmed.ncbi.nlm.nih.gov/34688610/
3. Long, Yingfei, Ren, Yifei, Wei, Qinglv, Cheng, Lei, Yi, Ping. 2023. NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression. In International journal of medical sciences, 20, 1079-1090. doi:10.7150/ijms.83828. https://pubmed.ncbi.nlm.nih.gov/37484809/
4. Mobet, Youchaou, Wang, Haocheng, Wei, Qinglv, Liu, Tao, Yi, Ping. 2024. AKAP8 promotes ovarian cancer progression and antagonizes PARP inhibitor sensitivity through regulating hnRNPUL1 transcription. In iScience, 27, 109744. doi:10.1016/j.isci.2024.109744. https://pubmed.ncbi.nlm.nih.gov/38711442/
5. Zhou, Yijie, Zhao, Keyuan, Li, Junlong, Xu, Gang, Pan, Shouhua. 2024. LINC00461 promotes bladder cancer cells EMT through miR-518b/HNRNPUL1 axis. In Discover oncology, 15, 419. doi:10.1007/s12672-024-01294-5. https://pubmed.ncbi.nlm.nih.gov/39254804/
6. Gurunathan, Gayathri, Yu, Zhenbao, Coulombe, Yan, Masson, Jean-Yves, Richard, Stéphane. 2015. Arginine methylation of hnRNPUL1 regulates interaction with NBS1 and recruitment to sites of DNA damage. In Scientific reports, 5, 10475. doi:10.1038/srep10475. https://pubmed.ncbi.nlm.nih.gov/26020839/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen