C57BL/6JCya-Camk2aem1/Cya
Common Name:
Camk2a-KO
Product ID:
S-KO-01308
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Camk2a-KO
Strain ID
KOCMP-12322-Camk2a-B6J-VA
Gene Name
Product ID
S-KO-01308
Gene Alias
CaMKII; mKIAA0968
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Camk2aem1/Cya mice (Catalog S-KO-01308) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102888
NCBI RefSeq
NM_177407
Target Region
Exon 2~16
Size of Effective Region
~39.7 kb
Detailed Document
Overview of Gene Research
Camk2a, also known as calcium/calmodulin-dependent protein kinase II alpha, is a key calcium signaling molecule. It is involved in the glutamatergic signaling pathway and plays essential roles in multiple biological processes. It has been shown to be crucial for normal learning and synaptic plasticity [1,2].
Mutations in Camk2a have been associated with intellectual disability. Rare de novo variants in Camk2a can affect its auto-phosphorylation at Thr286, which in turn impacts neuronal migration, highlighting the importance of tightly regulated auto-phosphorylation in neuronal function and neurodevelopment [1]. In a Camk2a knockout mouse model, adult reinstatement of the Camk2a gene can rescue behavioral and electrophysiological phenotypes, including spatial and conditional learning and synaptic plasticity, suggesting it does not have a critical irreversible role in neurodevelopment [2].
In summary, Camk2a is vital for normal brain function, especially in learning and synaptic plasticity. Studies using gene knockout mouse models have provided valuable insights into its role in intellectual disability and neurodevelopment, suggesting potential therapeutic implications for Camk2a-related disorders [1,2].
References:
1. Küry, Sébastien, van Woerden, Geeske M, Besnard, Thomas, Elgersma, Ype, Mercier, Sandra. . De Novo Mutations in Protein Kinase Genes CAMK2A and CAMK2B Cause Intellectual Disability. In American journal of human genetics, 101, 768-788. doi:10.1016/j.ajhg.2017.10.003. https://pubmed.ncbi.nlm.nih.gov/29100089/
2. Rigter, Pomme M F, Wallaard, Ilse, Aghadavoud Jolfaei, Mehrnoush, Elgersma, Ype, van Woerden, Geeske M. 2022. Adult Camk2a gene reinstatement restores the learning and plasticity deficits of Camk2a knockout mice. In iScience, 25, 105303. doi:10.1016/j.isci.2022.105303. https://pubmed.ncbi.nlm.nih.gov/36304100/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen