C57BL/6JCya-Cdkn1aem1/Cya
Common Name:
Cdkn1a-KO
Product ID:
S-KO-01466
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cdkn1a-KO
Strain ID
KOCMP-12575-Cdkn1a-B6J-VA
Gene Name
Product ID
S-KO-01466
Gene Alias
CAP20; CDKI; CIP1; Cdkn1; P21; SDI1; Waf1; mda6; p21Cip1; p21WAF
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdkn1aem1/Cya mice (Catalog S-KO-01466) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000119901
NCBI RefSeq
NM_001111099
Target Region
Exon 2
Size of Effective Region
~1.0kb
Detailed Document
Overview of Gene Research
Cdkn1a, also known as Cyclin-dependent kinase inhibitor 1A and with aliases such as Cip1/Waf1/p21, is a well-established protein. It is primarily recognized for its key role in the cell cycle, where it induces cell cycle arrest by inhibiting cyclin-dependent kinases (CDKs). Besides, it is involved in apoptosis, DNA damage response (DDR), and the regulation of stem cell fate [1].
In cisplatin-induced acute kidney injury (AKI), CDKN1A was identified as a key gene related to ferroptosis and the P53 pathway. Knockdown of CDKN1A in human renal tubular epithelial cells mitigated cisplatin-induced cell injury by reducing oxidative stress and ferroptosis, suggesting its potential as a biomarker for early diagnosis and a therapeutic target in AKI [2]. In esophageal cancer, CDKN1A polymorphisms were found to be associated with EC risk in relation to age. For example, certain genotypes and alleles were significantly associated with EC risk in different age groups [3]. In an Iranian population, the AC genotype of CDKN1A rs1801270 polymorphism may have a protective role in the development of colorectal cancer [4].
In conclusion, Cdkn1a is crucial for cell cycle regulation and is involved in multiple biological processes. Studies on Cdkn1a, especially through functional studies in relevant disease models like AKI and cancer, have revealed its significance in disease pathogenesis. These findings contribute to understanding the mechanisms of disease occurrence and may provide new directions for diagnosis and treatment.
References:
1. Manousakis, Evangelos, Miralles, Clàudia Martinez, Esquerda, Maria Guimerà, Wright, Roni H G. 2023. CDKN1A/p21 in Breast Cancer: Part of the Problem, or Part of the Solution? In International journal of molecular sciences, 24, . doi:10.3390/ijms242417488. https://pubmed.ncbi.nlm.nih.gov/38139316/
2. Gao, Qian, Chen, Jun-Ming, Li, Chen-Sui-Zi, Luo, Lian-Xiang, Li, Zhi-Ling. 2024. CDKN1A promotes Cis-induced AKI by inducing cytoplasmic ROS production and ferroptosis. In Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 193, 115003. doi:10.1016/j.fct.2024.115003. https://pubmed.ncbi.nlm.nih.gov/39353481/
3. Kaya, Zehra, Karan, Burak Mugdat, Almalı, Necat. 2021. CDKN1A (p21 gene) polymorphisms correlates with age in esophageal cancer. In Molecular biology reports, 49, 249-258. doi:10.1007/s11033-021-06865-1. https://pubmed.ncbi.nlm.nih.gov/34743275/
4. Zali, Neda, Savabkar, Sanaz, Tajali, Raziye, Hashemi, Mehrdad, Asadzadeh Aghdaei, Hamid. 2023. Association between CDKN1A gene rs1801270 polymorphisms and susceptibility to colorectal cancser in an Iranian population. In Nucleosides, nucleotides & nucleic acids, 42, 563-570. doi:10.1080/15257770.2023.2169934. https://pubmed.ncbi.nlm.nih.gov/36691945/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen