C57BL/6JCya-Chatem1/Cya
Common Name:
Chat-KO
Product ID:
S-KO-01494
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chat-KO
Strain ID
KOCMP-12647-Chat-B6J-VA
Gene Name
Product ID
S-KO-01494
Gene Alias
B230380D24Rik; CHOACTase
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chatem1/Cya mice (Catalog S-KO-01494) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070125
NCBI RefSeq
NM_009891
Target Region
Exon 3~7
Size of Effective Region
~9.8 kb
Detailed Document
Overview of Gene Research
Chat, or choline acetyltransferase, is a synthesizing enzyme for acetylcholine (ACh). ACh is a neurotransmitter involved in various physiological processes in both the central and peripheral nervous systems [1]. ChAT is produced in the neuron body, transported to the nerve terminal, and catalyzes the transfer of an acetyl group to choline to yield ACh. Genetic models, such as the ChAT-eGFP transgenic mouse, have provided advantages for studying cholinergic neurons [1].
In the ChAT-eGFP transgenic mouse study, while there was a complete overlap between the transgenic expression of eGFP and immunohistochemical staining in the cholinergic basal forebrain, differences were found in other areas like the cortex, hippocampus, and brainstem. Small neurons in the cortex and hippocampus showed discrepancies in eGFP expression and antibody labeling, and no eGFP expression was detected in certain cholinergic cells of the brainstem [1].
In conclusion, Chat is essential for the synthesis of acetylcholine, playing a crucial role in neural communication. The use of transgenic mouse models like the ChAT-eGFP has revealed both the widespread and specific distribution patterns of cholinergic neurons related to Chat, contributing to our understanding of neural function and potentially related neurological conditions.
References:
1. Gamage, Rashmi, Zaborszky, Laszlo, Münch, Gerald, Gyengesi, Erika. 2023. Evaluation of eGFP expression in the ChAT-eGFP transgenic mouse brain. In BMC neuroscience, 24, 4. doi:10.1186/s12868-023-00773-9. https://pubmed.ncbi.nlm.nih.gov/36650430/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen