C57BL/6JCya-Gabreem1/Cya
Common Name:
Gabre-KO
Product ID:
S-KO-02164
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gabre-KO
Strain ID
KOCMP-14404-Gabre-B6J-VA
Gene Name
Product ID
S-KO-02164
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gabreem1/Cya mice (Catalog S-KO-02164) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064780
NCBI RefSeq
NM_017369
Target Region
Exon 2~6
Size of Effective Region
~8.7 kb
Detailed Document
Overview of Gene Research
Gabre, encoding the GABA receptor ε -subunit, is expressed in a subset of preoptic area cell types in the hypothalamus. The GABA receptor is involved in neural inhibitory neurotransmission, and Gabre may confer resistance to anesthetics at the molecular level [1]. Understanding its function is crucial as the preoptic area is vital for regulating brain-body interactions, including control of vital signs [1].
A Gabre-cre knock-in mouse line was generated to interrogate the function of Gabre cells in the preoptic area [1]. In awake mice, chemogenetic activation of Gabre neurons in the preoptic area reduced body temperature, while chemogenetic inhibition of these neurons decreased the heart rate under isoflurane anesthesia [1]. This indicates an important role of preoptic Gabre neurons in maintaining vital signs like body temperature and heart rate during wakefulness and anesthesia [1].
In conclusion, Gabre is significant for its role in regulating vital signs such as body temperature and heart rate, as revealed through in vivo studies using the Gabre-cre knock-in mouse model. These findings contribute to our understanding of the physiological mechanisms related to anesthesia and maintenance of vital functions in the context of the preoptic area's neural circuits [1].
References:
1. Wang, Ziyue, Li, Lanxiang, Li, Miao, Naumann, Robert Konrad, Wang, Hong. 2024. Chemogenetic Modulation of Preoptic Gabre Neurons Decreases Body Temperature and Heart Rate. In International journal of molecular sciences, 25, . doi:10.3390/ijms252313061. https://pubmed.ncbi.nlm.nih.gov/39684772/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen