C57BL/6JCya-Jchainem1/Cya
Common Name
Jchain-KO
Product ID
S-KO-02605
Backgroud
C57BL/6JCya
Strain ID
KOCMP-16069-Jchain-B6J-VA
Status
When using this mouse strain in a publication, please cite “Jchain-KO Mouse (Catalog S-KO-02605) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Jchain-KO
Strain ID
KOCMP-16069-Jchain-B6J-VA
Gene Name
Product ID
S-KO-02605
Gene Alias
Igj, Jch, 9530090F24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000087033
NCBI RefSeq
NM_152839
Target Region
Exon 1~4
Size of Effective Region
~6.8 kb
Overview of Gene Research
Jchain, short for immunoglobulin joining chain, is essential for the assembly and transport of Immunoglobulin M (IgM) and Immunoglobulin A (IgA) in both humoral and mucosal immunity [1]. It binds to the tailpieces of IgM-Fc, stabilizing the IgM pentamer and bridging the interaction between IgM-Fc and the polymeric immunoglobulin receptor (pIgR), which is crucial for the function of IgM [1].
A mouse model expressing simian HBEGF (diphtheria toxin receptor, DTR) under the control of the endogenous Jchain locus (J-DTR) was developed [2]. In these mice, antibody-secreting cells (ASCs) expressed high levels of cell-surface DTR and were acutely depleted following diphtheria toxin treatment. This model allowed the tracking of ASC reconstitution following depletion in 3 distinct organs, providing a tool to study ASC biology [2].
In conclusion, Jchain is vital for the proper function of IgM in immunity. The J-DTR mouse model has been valuable in understanding the differentiation kinetics of antibody-secreting cells, contributing to the study of ASC biology. This genetic model-based research helps in revealing the role of Jchain in immune-related processes, potentially providing insights for diseases related to abnormal humoral and mucosal immunity [2].
References:
1. Li, Yaxin, Wang, Guopeng, Li, Ningning, Gao, Ning, Xiao, Junyu. 2020. Structural insights into immunoglobulin M. In Science (New York, N.Y.), 367, 1014-1017. doi:10.1126/science.aaz5425. https://pubmed.ncbi.nlm.nih.gov/32029689/
2. Pioli, KimAnh T, Ritchie, Matthew, Haq, Hira, Pioli, Peter D. 2024. Jchain-DTR Mice Allow for Diphtheria Toxin-Mediated Depletion of Antibody-Secreting Cells and Evaluation of Their Differentiation Kinetics. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.05.06.592703. https://pubmed.ncbi.nlm.nih.gov/38766257/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.