C57BL/6JCya-Mc4rem1/Cya
Common Name
Mc4r-KO
Product ID
S-KO-03151
Backgroud
C57BL/6JCya
Strain ID
KOCMP-17202-Mc4r-B6J-VA
Status
When using this mouse strain in a publication, please cite “Mc4r-KO Mouse (Catalog S-KO-03151) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mc4r-KO
Strain ID
KOCMP-17202-Mc4r-B6J-VA
Gene Name
Product ID
S-KO-03151
Gene Alias
Pkcp, Mc4-r
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 18
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000057942
NCBI RefSeq
NM_016977
Target Region
Exon 1
Size of Effective Region
~2.8 kb
Overview of Gene Research
MC4R, or melanocortin receptor-4, is a G protein-coupled receptor. It is involved in regulating food intake and energy metabolism through various signaling pathways, and is a key genetic factor in severe and early-onset obesity and hyperphagia disorders [1]. MC4R is part of the leptin-melanocortin pathway, which is crucial for bodyweight regulation [4].
In rodent studies, wild-type rodents are more sensitive to the MC4R agonist Setmelanotide compared to MC4R heterozygous deficient mice, while MC4R knockout mice fail to respond, indicating the role of MC4R in weight loss response to the agonist [3]. Also, in a Phase 1b clinical trial, Setmelanotide led to weight loss in obese MC4R variant carriers [3].
In conclusion, MC4R plays a vital role in energy metabolism and bodyweight regulation. Studies using rodent models, especially knockout models, have been instrumental in understanding its function. These findings have implications for developing treatments for obesity and related cardiometabolic diseases, as targeting MC4R-related pathways may offer effective strategies for weight loss and disease management [2,3].
References:
1. Wei, Ran, Li, Danjie, Jia, Sheng, Chen, Yuhong, Wang, Jiqiu. 2023. MC4R in Central and Peripheral Systems. In Advanced biology, 7, e2300035. doi:10.1002/adbi.202300035. https://pubmed.ncbi.nlm.nih.gov/37043700/
2. Lotta, Luca A, Mokrosiński, Jacek, Mendes de Oliveira, Edson, Wareham, Nicholas J, Farooqi, I Sadaf. . Human Gain-of-Function MC4R Variants Show Signaling Bias and Protect against Obesity. In Cell, 177, 597-607.e9. doi:10.1016/j.cell.2019.03.044. https://pubmed.ncbi.nlm.nih.gov/31002796/
3. Collet, Tinh-Hai, Dubern, Béatrice, Mokrosinski, Jacek, Farooqi, I Sadaf, Van der Ploeg, Lex H T. 2017. Evaluation of a melanocortin-4 receptor (MC4R) agonist (Setmelanotide) in MC4R deficiency. In Molecular metabolism, 6, 1321-1329. doi:10.1016/j.molmet.2017.06.015. https://pubmed.ncbi.nlm.nih.gov/29031731/
4. Clément, Karine, van den Akker, Erica, Argente, Jesús, Wabitsch, Martin, Kühnen, Peter. 2020. Efficacy and safety of setmelanotide, an MC4R agonist, in individuals with severe obesity due to LEPR or POMC deficiency: single-arm, open-label, multicentre, phase 3 trials. In The lancet. Diabetes & endocrinology, 8, 960-970. doi:10.1016/S2213-8587(20)30364-8. https://pubmed.ncbi.nlm.nih.gov/33137293/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.