C57BL/6JCya-Nedd8em1/Cya
Common Name
Nedd8-KO
Product ID
S-KO-03347
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18002-Nedd8-B6J-VA
When using this mouse strain in a publication, please cite “Nedd8-KO Mouse (Catalog S-KO-03347) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nedd8-KO
Strain ID
KOCMP-18002-Nedd8-B6J-VA
Gene Name
Product ID
S-KO-03347
Gene Alias
NEDD-8, Rub1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000010520
NCBI RefSeq
NM_008683.4
Target Region
Exon 2~3
Size of Effective Region
~445 bp
Overview of Gene Research
Nedd8, a ubiquitin-like protein, plays a crucial role in the regulation of diverse biological processes. It is tightly associated with the cullin-RING E3 ligase (CRL)-catalysed protein ubiquitylation pathway. Neddylation, the process of attaching Nedd8 to target proteins, is essential for the proper functioning of CRLs, which are involved in many cellular processes such as cell cycle regulation, DNA repair, and signal transduction [1,3].
In triple-negative breast cancer (TNBC), loss-of-function studies using CRISPR/Cas9 screens revealed that deletion of NEDD8 in cancer cells initially delays cell division, but cell proliferation recovers. However, it causes vulnerability to nivolumab (anti-PD-1), indicating NEDD8 as a potential synergistic drug target for PD-1/L1 blockade therapy. This shows how NEDD8 is conditionally essential in TNBC [2].
In conclusion, Nedd8 is vital for the regulation of CRL-mediated protein ubiquitylation, influencing numerous cellular processes. The discovery of its role in conferring vulnerability to immune checkpoint blockade in TNBC through loss-of-function models highlights its potential as a therapeutic target in this disease area.
References:
1. Baek, Kheewoong, Krist, David T, Prabu, J Rajan, Kleiger, Gary, Schulman, Brenda A. 2020. NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly. In Nature, 578, 461-466. doi:10.1038/s41586-020-2000-y. https://pubmed.ncbi.nlm.nih.gov/32051583/
2. Papakyriacou, Irineos, Kutkaite, Ginte, Rúbies Bedós, Marta, Menden, Michael P, Mao, Yumeng. 2024. Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer. In Nature communications, 15, 3581. doi:10.1038/s41467-024-47987-x. https://pubmed.ncbi.nlm.nih.gov/38678024/
3. Schwechheimer, Claus. 2018. NEDD8-its role in the regulation of Cullin-RING ligases. In Current opinion in plant biology, 45, 112-119. doi:10.1016/j.pbi.2018.05.017. https://pubmed.ncbi.nlm.nih.gov/29909289/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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