C57BL/6JCya-Emc8em1/Cya
Common Name
Emc8-KO
Product ID
S-KO-03399
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18117-Emc8-B6J-VA
When using this mouse strain in a publication, please cite “Emc8-KO Mouse (Catalog S-KO-03399) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Emc8-KO
Strain ID
KOCMP-18117-Emc8-B6J-VA
Gene Name
Product ID
S-KO-03399
Gene Alias
Cox4nb, Fam158b, Noc4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000034277
NCBI RefSeq
NM_010926
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Overview of Gene Research
Emc8, a component of the endoplasmic reticulum membrane protein complex (EMC), is crucial for the insertion of diverse transmembrane proteins into the plasma membrane [2]. Each EMC consists of Emc1-7, Emc10, along with either Emc8 or Emc9. The EMC is involved in the biogenesis of multi-pass transmembrane proteins, which is vital for normal cellular function [3]. Genetic models, such as Drosophila models, have been used to study its function [3].
In a study on systemic lupus erythematosus (SLE), the methylation level in the promoter region of Emc8 increased after fecal microbiota transplantation (FMT) in the responder group, indicating a potential link between FMT-induced methylation changes and the disease mechanism [1].
In conclusion, Emc8 is essential for the proper insertion and biogenesis of transmembrane proteins. The discovery of its promoter methylation change in SLE patients after FMT through model-based research provides insights into the role of Emc8 in this autoimmune disease, potentially offering new directions for understanding the disease mechanism and developing treatment strategies.
References:
1. Zhang, Bo, Zhou, Wenhui, Liu, Qianmei, Zhao, Ming, Lu, Qianjin. 2023. Effects of fecal microbiota transplant on DNA methylation in patients with systemic lupus erythematosus. In Journal of autoimmunity, 141, 103047. doi:10.1016/j.jaut.2023.103047. https://pubmed.ncbi.nlm.nih.gov/37179169/
2. Marquez, Jonathan, Aslam, Faiza, Khokha, Mustafa K. 2023. Expanding EMC foldopathies: Topogenesis deficits alter the neural crest. In Genesis (New York, N.Y. : 2000), 61, e23520. doi:10.1002/dvg.23520. https://pubmed.ncbi.nlm.nih.gov/37318954/
3. Satoh, Takunori, Ohba, Aya, Liu, Ziguang, Inagaki, Tsuyoshi, Satoh, Akiko K. 2015. dPob/EMC is essential for biosynthesis of rhodopsin and other multi-pass membrane proteins in Drosophila photoreceptors. In eLife, 4, . doi:10.7554/eLife.06306. https://pubmed.ncbi.nlm.nih.gov/25715730/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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