C57BL/6JCya-Per2em1/Cya
Common Name:
Per2-KO
Product ID:
S-KO-03621
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Per2-KO
Strain ID
KOCMP-18627-Per2-B6J-VA
Gene Name
Product ID
S-KO-03621
Gene Alias
mKIAA0347; mPer2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Per2em1/Cya mice (Catalog S-KO-03621) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069620
NCBI RefSeq
NM_011066
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Per2, short for Period2, is a core component of the circadian rhythm, playing a vital role in regulating the circadian rhythm of the biological clock [3]. It is involved in various pathways and biological processes, regulating multiple physiological systems like metabolism, sleep, and immune function [4]. Genetic models, such as the PER2::LUC knockin mouse, have been crucial for studying its spatiotemporal patterns in the suprachiasmatic nuclei [5].
In disease-related research, in oral squamous cell carcinoma (OSCC), PER2 expression is reduced. Overexpression of PER2 inhibits PD-L1 expression by suppressing the IKK/NF-κB pathway, enhancing T-cell-mediated OSCC cell killing [1]. In pituitary adenomas, PER2 is prominently upregulated. Jetlagged mice with PER2 upregulation have accelerated growth of GH3 xenograft tumors, while loss of Per2 protects mice against developing estrogen-induced pituitary adenoma, suggesting PER2 promotes pituitary tumorigenesis by regulating cell cycle genes [2]. In ulcerative colitis, PER2 is decreased in inflamed mucosa and peripheral blood CD4+ T cells of patients. Overexpression of PER2 in UC CD4+ T cells inhibits IFN-γ production by down-regulating ADAM12 [6].
In conclusion, Per2 is essential for regulating the circadian rhythm and is involved in multiple biological processes. Through gene knockout and other model-based research, it has been shown to play significant roles in diseases such as OSCC, pituitary adenomas, and ulcerative colitis, providing potential therapeutic targets for these conditions.
References:
1. Zhang, Zhiwei, Sun, Deping, Tang, Hong, Yin, Shilin, Yang, Kai. . PER2 binding to HSP90 enhances immune response against oral squamous cell carcinoma by inhibiting IKK/NF-κB pathway and PD-L1 expression. In Journal for immunotherapy of cancer, 11, . doi:10.1136/jitc-2023-007627. https://pubmed.ncbi.nlm.nih.gov/37914384/
2. Guo, Lianxia, Cen, Haobin, Weng, Jiaxian, Chen, Zhiyong, Wu, Baojian. 2023. PER2 integrates circadian disruption and pituitary tumorigenesis. In Theranostics, 13, 2657-2672. doi:10.7150/thno.82995. https://pubmed.ncbi.nlm.nih.gov/37215573/
3. Jiang, Huihui, Yang, Xin, Mi, Miaomiao, Xin, Yu, Sun, Chengming. 2021. PER2: a potential molecular marker for hematological malignancies. In Molecular biology reports, 48, 7587-7595. doi:10.1007/s11033-021-06751-w. https://pubmed.ncbi.nlm.nih.gov/34642831/
4. Pavithra, S, Aich, Adrija, Chanda, Adrita, Gawade, Pranotee, Das, Raunak Kumar. 2023. PER2 gene and its association with sleep-related disorders: A review. In Physiology & behavior, 273, 114411. doi:10.1016/j.physbeh.2023.114411. https://pubmed.ncbi.nlm.nih.gov/37981094/
5. Leise, Tanya L. . Computational Analysis of PER2::LUC Imaging Data. In Methods in molecular biology (Clifton, N.J.), 2130, 295-302. doi:10.1007/978-1-0716-0381-9_22. https://pubmed.ncbi.nlm.nih.gov/33284453/
6. Ye, Yulan, Liu, Changqin, Wu, Ruijin, Liu, Zhanju, Chen, Liang. 2024. Circadian clock component PER2 negatively regulates CD4+ T cell IFN-γ production in ulcerative colitis. In Mucosal immunology, 17, 1161-1173. doi:10.1016/j.mucimm.2024.07.010. https://pubmed.ncbi.nlm.nih.gov/39097147/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen