C57BL/6JCya-Tmem132bem1/Cya
Common Name
Tmem132b-KO
Product ID
S-KO-04531
Backgroud
C57BL/6JCya
Strain ID
KOCMP-208151-Tmem132b-B6J-VA
When using this mouse strain in a publication, please cite “Tmem132b-KO Mouse (Catalog S-KO-04531) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tmem132b-KO
Strain ID
KOCMP-208151-Tmem132b-B6J-VA
Gene Name
Product ID
S-KO-04531
Gene Alias
mKIAA1786
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031446
NCBI RefSeq
NM_001190352
Target Region
Exon 2
Size of Effective Region
~3.1 kb
Overview of Gene Research
TMEM132B, a transmembrane protein, functions as a GABAA receptor (GABAAR) auxiliary subunit. It promotes GABAAR expression at the cell surface, slows receptor deactivation, and enhances alcohol's allosteric effects on the receptor. The TMEM132B-GABAAR complex is involved in GABAergic transmission pathways, and its function is crucial for understanding alcohol-related behaviors [1].
In TMEM132B knockout (KO) or TMEM132B I499A knockin (KI) mice where the TMEM132B-GABAAR interaction is abolished, GABAergic transmission in hippocampal neurons is decreased. Alcohol-induced potentiation of GABAAR-mediated currents is also diminished. Behaviorally, the anxiolytic and sedative/hypnotic effects of alcohol are reduced, while compulsive, binge-like alcohol consumption is increased. These findings from KO and KI mouse models reveal that TMEM132B is key in mediating alcohol-related behaviors in the brain [1].
In conclusion, TMEM132B plays a vital role in regulating GABAergic transmission and alcohol-related behaviors as demonstrated by mouse model-based research. Understanding TMEM132B function through these models provides insights into the molecular mechanisms of alcohol-related behaviors, potentially offering new strategies for addressing alcohol-related disorders.
References:
1. Wang, Guohao, Peng, Shixiao, Reyes Mendez, Miriam, Li, Yan, Lu, Wei. 2024. The TMEM132B-GABAA receptor complex controls alcohol actions in the brain. In Cell, 187, 6649-6668.e35. doi:10.1016/j.cell.2024.09.006. https://pubmed.ncbi.nlm.nih.gov/39357522/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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