C57BL/6JCya-Tsnem1/Cya
Common Name
Tsn-KO
Product ID
S-KO-05579
Backgroud
C57BL/6JCya
Strain ID
KOCMP-22099-Tsn-B6J-VA
When using this mouse strain in a publication, please cite “Tsn-KO Mouse (Catalog S-KO-05579) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tsn-KO
Strain ID
KOCMP-22099-Tsn-B6J-VA
Gene Name
Product ID
S-KO-05579
Gene Alias
C3PO, TB-RBP, 2610034C24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000027623
NCBI RefSeq
NM_011650
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Overview of Gene Research
Tsn, or Tudor-staphylococcal/micrococcal-like nuclease, is involved in the cellular pathway of miRNA decay known as TumiD. It promotes the decay of miRNAs containing CA and/or UA dinucleotides, which is important for regulating the expression of protein-encoding transcripts, thus impacting various biological processes [1].
In T24 human urinary bladder cancer cells, the ATP-dependent RNA helicase UPF1 was found to be required for cellular TumiD. UPF1 can dissociate miRNAs from their mRNA targets, making them more susceptible to TSN-mediated degradation. This augmentation of TumiD by UPF1 promotes the invasion of T24 cells in part by degrading anti-invasive miRNAs and up-regulating pro-invasive proteins [1].
In conclusion, Tsn is a key nuclease in miRNA decay, with its function in this process being influenced by UPF1. The study of Tsn through in vitro and in vivo models, such as in T24 human urinary bladder cancer cells, helps to understand its role in cellular invasion, providing insights into cancer-related biological processes and potentially new strategies for cancer treatment.
References:
1. Elbarbary, Reyad A, Miyoshi, Keita, Hedaya, Omar, Myers, Jason R, Maquat, Lynne E. 2017. UPF1 helicase promotes TSN-mediated miRNA decay. In Genes & development, 31, 1483-1493. doi:10.1101/gad.303537.117. https://pubmed.ncbi.nlm.nih.gov/28827400/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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