C57BL/6JCya-Thrap3em1/Cya
Common Name
Thrap3-KO
Product ID
S-KO-06366
Backgroud
C57BL/6JCya
Strain ID
KOCMP-230753-Thrap3-B6J-VA
When using this mouse strain in a publication, please cite “Thrap3-KO Mouse (Catalog S-KO-06366) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Thrap3-KO
Strain ID
KOCMP-230753-Thrap3-B6J-VA
Gene Name
Product ID
S-KO-06366
Gene Alias
9330151F09Rik, B230333E16Rik, Trap150
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000080919
NCBI RefSeq
NM_146153
Target Region
Exon 2~4
Size of Effective Region
~8.7 kb
Overview of Gene Research
Thrap3, also known as thyroid hormone receptor-associated protein 3, is involved in multiple biological processes and associated pathways. It participates in transcriptional regulation, interacting with various proteins, and is important for maintaining normal cellular functions, with implications in metabolic regulation, osteogenesis, and genomic stability [1-3]. Gene knockout (KO) and conditional knockout (CKO) mouse models have been valuable in studying Thrap3's functions.
In a high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) model, liver-specific Thrap3 knockout improved lipid accumulation and metabolic properties. Thrap3 deficiency enhanced autophagy and mitochondrial function, and knockout increased the cytosolic translocation and activation of AMPK, indicating its role in NAFLD progression [1]. In the context of osteogenesis, Thrap3 promoted osteoblast differentiation through the Thrap3-Sox9-Runx2 axis by inhibiting the degradation of Runx2 [2]. In cancer cells, Thrap3 interacted with methylated DDX5 to promote R-loop resolution, and its loss increased R-loop accumulation and DNA damage [3].
In conclusion, Thrap3 is crucial in multiple biological processes. KO/CKO mouse models have revealed its role in NAFLD, osteogenesis, and cancer-related genomic stability. Understanding Thrap3 functions provides potential therapeutic targets for diseases such as NAFLD, osteoporosis, and cancer [1-3].
References:
1. Jang, Hyun-Jun, Lee, Yo Han, Dao, Tam, Park, Sung Ho, Choi, Jang Hyun. 2023. Thrap3 promotes nonalcoholic fatty liver disease by suppressing AMPK-mediated autophagy. In Experimental & molecular medicine, 55, 1720-1733. doi:10.1038/s12276-023-01047-4. https://pubmed.ncbi.nlm.nih.gov/37524868/
2. Zhou, Lei, Li, Haoran, Sun, Shiwei, Xu, Liang, Wang, Minghai. . Thrap3 promotes osteogenesis by inhibiting the degradation of Runx2. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22231. doi:10.1096/fj.202101706R. https://pubmed.ncbi.nlm.nih.gov/35230719/
3. Kang, Hyun Je, Eom, Hye-Jin, Kim, Hongtae, Kwon, Hyug Moo, Choi, Jang Hyun. 2021. Thrap3 promotes R-loop resolution via interaction with methylated DDX5. In Experimental & molecular medicine, 53, 1602-1611. doi:10.1038/s12276-021-00689-6. https://pubmed.ncbi.nlm.nih.gov/34697388/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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