C57BL/6JCya-Pcyox1lem1/Cya
Common Name:
Pcyox1l-KO
Product ID:
S-KO-07185
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pcyox1l-KO
Strain ID
KOCMP-240334-Pcyox1l-B6J-VA
Gene Name
Product ID
S-KO-07185
Gene Alias
C630049M13
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pcyox1lem1/Cya mice (Catalog S-KO-07185) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025472
NCBI RefSeq
NM_172832
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Pcyox1l, or Prenylcysteine oxidase 1 like protein, is significantly conserved evolutionarily, with murine and human proteins sharing 94% amino-acid homology. It is involved in modulating the prenylation pathway, which impacts autophagy, cellular viability, and has connections to neutrophil functionality [1].
In gene-knockout studies, loss of Pcyox1l protein leads to significant reductions in the mevalonate pathway, affecting autophagy and cellular viability under homeostatic conditions. Pcyox1l CRISPR-edited neutrophils show deficient bactericidal properties, and Pcyox1l knockout mice are highly susceptible to infection by the gram-negative pathogen Pseudomonas aeruginosa, as evidenced by increased neutrophil infiltrates, hemorrhaging, and reduced bactericidal functionality [1]. In addition, in a deep vein thrombosis (DVT) mouse model, lncRNA Crnde promotes DVT by sequestering miR-181a-5p, which in turn releases the expression of Pcyox1l, aggravating thrombus formation [2].
In conclusion, Pcyox1l is crucial for neutrophil bactericidal activities through its role in the prenylation pathway. Gene knockout mouse models have revealed its significance in infection susceptibility. Additionally, its involvement in the lncRNA-miRNA-mRNA ceRNA network in DVT further highlights its importance in disease-related biological processes [1,2].
References:
1. Petenkova, Anastasiia, Auger, Shelby A, Lamb, Jeffrey, Distefano, Mark D, Gadjeva, Mihaela. 2023. Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities. In Nature communications, 14, 2761. doi:10.1038/s41467-023-38447-z. https://pubmed.ncbi.nlm.nih.gov/37179332/
2. He, Xin, Liu, Yu, Li, Yaozhen, Wu, Kemin. 2023. Long non-coding RNA crnde promotes deep vein thrombosis by sequestering miR-181a-5p away from thrombogenic Pcyox1l. In Thrombosis journal, 21, 44. doi:10.1186/s12959-023-00480-9. https://pubmed.ncbi.nlm.nih.gov/37076891/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen