C57BL/6NCya-Il27em1/Cya
Common Name
Il27-KO
Product ID
S-KO-07663
Backgroud
C57BL/6NCya
Strain ID
KOCMP-246779-Il27-B6N-VA
When using this mouse strain in a publication, please cite “Il27-KO Mouse (Catalog S-KO-07663) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Il27-KO
Strain ID
KOCMP-246779-Il27-B6N-VA
Gene Name
Product ID
S-KO-07663
Gene Alias
p28, Il30, IL-27, IL27-A, IL-27-A, IL-27p28
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000058429
NCBI RefSeq
NM_145636
Target Region
Exon 2~4
Size of Effective Region
~1.9 kb
Overview of Gene Research
Il27, interleukin-27, is a cytokine with diverse influences on the immune response. It was initially linked to Th1 responses, but now is recognized as a modulator of T cell effector functions, inducer of IL-10, and promoter of T regulatory cell responses. It is involved in multiple biological processes, especially those related to immunity, metabolism, and cancer [2].
In hepatocellular carcinoma (HCC), loss-of-function experiments in two different models of hepatocarcinogenesis showed that loss of Il27R suppressed HCC in vivo. Il27R signaling within the tumor microenvironment restrains the cytotoxicity of innate cytotoxic lymphocytes, and its ablation enhanced their accumulation and activation [1]. In melanoma, high Il27 expression was associated with improved survival, and it may drive CD8+ T cell infiltration by suppressing β-catenin signaling in the tumor microenvironment [3].
In conclusion, Il27 plays crucial roles in immunity and cancer. Gene knockout or conditional knockout mouse models have revealed its function in promoting HCC development by regulating innate cytotoxic lymphocytes, and its potential beneficial role in melanoma prognosis. These findings suggest its potential as a therapeutic target in these diseases [1,3].
References:
1. Aghayev, Turan, Mazitova, Aleksandra M, Fang, Jennifer R, Grivennikov, Sergei I, Koltsova, Ekaterina K. . IL27 Signaling Serves as an Immunologic Checkpoint for Innate Cytotoxic Cells to Promote Hepatocellular Carcinoma. In Cancer discovery, 12, 1960-1983. doi:10.1158/2159-8290.CD-20-1628. https://pubmed.ncbi.nlm.nih.gov/35723626/
2. Yoshida, Hiroki, Hunter, Christopher A. . The immunobiology of interleukin-27. In Annual review of immunology, 33, 417-43. doi:10.1146/annurev-immunol-032414-112134. https://pubmed.ncbi.nlm.nih.gov/25861977/
3. Dong, Chunyu, Dang, Dan, Zhao, Xuesong, Wang, Zhijun, Zhang, Chuan. 2021. Integrative Characterization of the Role of IL27 In Melanoma Using Bioinformatics Analysis. In Frontiers in immunology, 12, 713001. doi:10.3389/fimmu.2021.713001. https://pubmed.ncbi.nlm.nih.gov/34733272/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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