C57BL/6JCya-Nopchap1em1/Cya
Common Name
Nopchap1-KO
Product ID
S-KO-09008
Backgroud
C57BL/6JCya
Strain ID
KOCMP-28109-Nopchap1-B6J-VA
When using this mouse strain in a publication, please cite “Nopchap1-KO Mouse (Catalog S-KO-09008) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nopchap1-KO
Strain ID
KOCMP-28109-Nopchap1-B6J-VA
Gene Name
Product ID
S-KO-09008
Gene Alias
C430041I18Rik, D10Wsu102e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020488
NCBI RefSeq
NM_026579
Target Region
Exon 1~4
Size of Effective Region
~128.3 kb
Overview of Gene Research
Nopchap1, also known as C12ORF45, is a PAQosome cofactor. The PAQosome, composed of the HSP90/R2TP chaperone and a prefoldin-like module, promotes the biogenesis of cellular machineries. Nopchap1 plays a crucial role in box C/D snoRNP biogenesis by acting as a bridge between NOP58 and PAQosome, and its study can be facilitated by genetic models [1].
In the research, Nopchap1 was found to directly interact with the CC-NOP domain of NOP58 and domain II of RUVBL1/2 AAA+ ATPases. Its interaction with RUVBL1/2 is disrupted upon ATP binding. In NOPCHAP1 KO cells, the expression of NOP58, but not NOP56 or PRPF31, is decreased, indicating that Nopchap1 is a client-loading PAQosome cofactor that selects NOP58 to promote box C/D snoRNP assembly [1]. Additionally, in a GWAS on Duroc × (Landrace × Yorkshire) pigs, Nopchap1 was identified as one of the 12 promising candidate genes associated with four bone weight traits, suggesting its potential role in pig bone weight-related genetic mechanisms [2].
In conclusion, Nopchap1 is essential for box C/D snoRNP assembly through its role as a PAQosome cofactor. The NOPCHAP1 KO cell experiments have revealed its specific function in this process. Moreover, its identification in pig GWAS implies a possible role in bone-related biological processes. These findings contribute to understanding its diverse biological functions and potential significance in related disease or trait-associated areas [1,2].
References:
1. Abel, Yoann, Paiva, Ana C F, Bizarro, Jonathan, Bertrand, Edouard, Verheggen, Céline. . NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis. In Nucleic acids research, 49, 1094-1113. doi:10.1093/nar/gkaa1226. https://pubmed.ncbi.nlm.nih.gov/33367824/
2. Gao, Xin, Zhou, Shenping, Liu, Zhihong, Wu, Zhenfang, Yang, Ming. 2023. Genome-Wide Association Study for Somatic Skeletal Traits in Duroc × (Landrace × Yorkshire) Pigs. In Animals : an open access journal from MDPI, 14, . doi:10.3390/ani14010037. https://pubmed.ncbi.nlm.nih.gov/38200769/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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