Gm5532 is a long noncoding RNA (lncRNA) with significant importance in bone metabolism. It functions as a regulator in osteoclast-related processes, and is involved in the miR-125a-3p/TRAF6 axis, which is crucial for understanding its role in bone-related biological pathways [1].

lncRNA microarray analysis identified Gm5532 as highly expressed during osteoclast differentiation. Knockdown of lncRNA-Gm5532 impairs osteoclast formation and bone resorption. Mechanistically, it acts as a competing endogenous RNA (ceRNA), sponging miR-125a-3p, thus promoting TNF receptor-associated factor 6 (TRAF6) expression. miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling, while its inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation [1].

In conclusion, Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis. The study of Gm5532 knockdown in the context of osteoclast differentiation reveals its potential as a novel biomarker and therapeutic target for osteoporosis [1].

References:

1. Zhang, Jian, Zhang, Lingyan, Yao, Gang, Qiao, Penghai, Wu, Shuguang. . lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis. In Acta biochimica et biophysica Sinica, 56, 54-61. doi:10.3724/abbs.2023245. https://pubmed.ncbi.nlm.nih.gov/38098360/