C57BL/6NCya-Zdhhc12em1/Cya
Common Name
Zdhhc12-KO
Product ID
S-KO-11597
Backgroud
C57BL/6NCya
Strain ID
KOCMP-66220-Zdhhc12-B6N-VA
When using this mouse strain in a publication, please cite “Zdhhc12-KO Mouse (Catalog S-KO-11597) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Zdhhc12-KO
Strain ID
KOCMP-66220-Zdhhc12-B6N-VA
Gene Name
Product ID
S-KO-11597
Gene Alias
1190004A01Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000081838
NCBI RefSeq
NM_025428
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Overview of Gene Research
Zdhhc12, a zinc finger DHHC-type palmitoyltransferase, plays a crucial role in protein S-palmitoylation, a post-translational modification. This modification is involved in diverse biological processes such as regulating protein stability, localization, and function within cells. It is associated with pathways related to inflammation, tumorigenesis, and innate immune responses [1-10]. Genetic models, like gene knockout (KO) mouse models, are valuable tools to study Zdhhc12's functions.
In KO mouse models, Zdhhc12 deficiency enhances inflammatory symptoms and lethality following alum-induced peritonitis and LPS-induced endotoxic shock, indicating its role in suppressing NLRP3 inflammasome-mediated inflammation [2]. In ovarian cancer, knockdown of Zdhhc12 inhibits claudin-3 (CLDN3) accurate membrane localization, protein stability, and ovarian cancer cell tumorigenesis, suggesting it promotes ovarian cancer progression through CLDN3 S-palmitoylation [1]. Also, in glioma cells, knockdown of Zdhhc12 reduces cell growth, migration, and invasion, showing its contribution to glioma malignancy [3].
In conclusion, Zdhhc12 is essential for regulating protein function through S-palmitoylation, influencing processes like inflammation and tumorigenesis. Studies using KO/CKO mouse models have revealed its role in inflammatory diseases such as sepsis-related inflammation and in cancer types like ovarian cancer and glioma, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Yuan, Meng, Chen, Xiaobing, Sun, Yitang, Cao, Ji, He, Qiaojun. 2020. ZDHHC12-mediated claudin-3 S-palmitoylation determines ovarian cancer progression. In Acta pharmaceutica Sinica. B, 10, 1426-1439. doi:10.1016/j.apsb.2020.03.008. https://pubmed.ncbi.nlm.nih.gov/32963941/
2. Wang, Liqiu, Cai, Jing, Zhao, Xin, Jin, Shouheng, Cui, Jun. 2022. Palmitoylation prevents sustained inflammation by limiting NLRP3 inflammasome activation through chaperone-mediated autophagy. In Molecular cell, 83, 281-297.e10. doi:10.1016/j.molcel.2022.12.002. https://pubmed.ncbi.nlm.nih.gov/36586411/
3. Lu, Feng, Shen, Shang-Hang, Wu, Shizhong, Zeng, Guangming, Wei, De. . Hypomethylation-induced prognostic marker zinc finger DHHC-type palmitoyltransferase 12 contributes to glioblastoma progression. In Annals of translational medicine, 10, 334. doi:10.21037/atm-22-520. https://pubmed.ncbi.nlm.nih.gov/35434031/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.