C57BL/6NCya-Rnmtem1/Cya
Common Name:
Rnmt-KO
Product ID:
S-KO-12510
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rnmt-KO
Strain ID
KOCMP-67897-Rnmt-B6N-VA
Gene Name
Product ID
S-KO-12510
Gene Alias
2610002P10Rik; Rg7mt1; mKIAA0398
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rnmtem1/Cya mice (Catalog S-KO-12510) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009679
NCBI RefSeq
NM_026440
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Rnmt, short for RNA guanine-7 methyltransferase, is a crucial enzyme in eukaryotic gene expression. It catalyzes the N7 methylation of the cap guanosine in the mRNA cap formation process, which is essential for mRNA processing, translation initiation, and nuclear RNA export [4,5,6]. This process is involved in various biological pathways such as cell differentiation, proliferation, and ribosome biogenesis [1,2].
In T cell activation, Rnmt is induced by TCR stimulation and coordinates the production of mRNA, snoRNA, and rRNA required for ribosome biogenesis. Rnmt cKO CD4 T cells show decreased ribosome synthesis, reduced translation rates, and proliferation failure, indicating its essential role in T cell activation [2]. In osteosarcoma, Rnmt collaborates with CYFIP1 to induce the m7G methylation of AURKAIP1 mRNA, leading to cuproptosis in OS cells, thus suppressing OS occurrence and progression [3]. In breast cancer cells, oncogenic PIK3CA mutations increase the dependency on Rnmt for proliferation. When Rnmt activity is reduced, a subset of breast cancer cells with PIK3CA mutations exhibit reduced proliferation and increased apoptosis [7].
In conclusion, Rnmt is vital for mRNA cap methylation, which has far-reaching impacts on gene expression and various biological processes. Through gene knockout (KO) or conditional knockout (CKO) mouse models, its role in diseases like T cell-related disorders, osteosarcoma, and breast cancer has been revealed, providing potential therapeutic targets for these diseases.
References:
1. Liang, Shang, Almohammed, Rajaei, Cowling, Victoria H. . The RNA cap methyltransferases RNMT and CMTR1 co-ordinate gene expression during neural differentiation. In Biochemical Society transactions, 51, 1131-1141. doi:10.1042/BST20221154. https://pubmed.ncbi.nlm.nih.gov/37145036/
2. Galloway, Alison, Kaskar, Aneesa, Ditsova, Dimitrinka, Jemielity, Jacek, Cowling, Victoria H. . Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation. In Nucleic acids research, 49, 6722-6738. doi:10.1093/nar/gkab465. https://pubmed.ncbi.nlm.nih.gov/34125914/
3. Lin, Zili, Wu, Ziyi, He, Yizhe, Li, Xiangyao, Luo, Wei. 2025. CYFIP1 coordinate with RNMT to induce osteosarcoma cuproptosis via AURKAIP1 m7G modification. In Molecular medicine (Cambridge, Mass.), 31, 74. doi:10.1186/s10020-025-01127-3. https://pubmed.ncbi.nlm.nih.gov/39984834/
4. Aregger, Michael, Kaskar, Aneesa, Varshney, Dhaval, Weidlich, Simone, Cowling, Victoria H. . CDK1-Cyclin B1 Activates RNMT, Coordinating mRNA Cap Methylation with G1 Phase Transcription. In Molecular cell, 61, 734-746. doi:10.1016/j.molcel.2016.02.008. https://pubmed.ncbi.nlm.nih.gov/26942677/
5. Osborne, Michael J, Volpon, Laurent, Memarpoor-Yazdi, Mina, Cowling, Victoria H, Borden, Katherine L B. 2022. Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E. In Journal of molecular biology, 434, 167451. doi:10.1016/j.jmb.2022.167451. https://pubmed.ncbi.nlm.nih.gov/35026230/
6. Aregger, Michael, Cowling, Victoria H. . Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites. In The Biochemical journal, 455, 67-73. doi:10.1042/BJ20130378. https://pubmed.ncbi.nlm.nih.gov/23863084/
7. Dunn, Sianadh, Lombardi, Olivia, Lukoszek, Radoslaw, Cowling, Victoria H. . Oncogenic PIK3CA mutations increase dependency on the mRNA cap methyltransferase, RNMT, in breast cancer cells. In Open biology, 9, 190052. doi:10.1098/rsob.190052. https://pubmed.ncbi.nlm.nih.gov/30991934/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen