C57BL/6JCya-Trmoem1/Cya
Common Name
Trmo-KO
Product ID
S-KO-14512
Backgroud
C57BL/6JCya
Strain ID
KOCMP-74753-Trmo-B6J-VA
When using this mouse strain in a publication, please cite “Trmo-KO Mouse (Catalog S-KO-14512) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Trmo-KO
Strain ID
KOCMP-74753-Trmo-B6J-VA
Gene Name
Product ID
S-KO-14512
Gene Alias
5830415F09Rik, Nap1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000086563
NCBI RefSeq
NM_029086
Target Region
Exon 2~5
Size of Effective Region
~7.7 kb
Overview of Gene Research
TrmO, previously known as YaeB, is a tRNA methyltransferase. It belongs to a novel category of AdoMet-dependent methyltransferases (Class VIII). Its main function is to catalyze the N(6)-methylation of N6-threonylcarbamoyladenosine (m(6)t(6)A) in tRNA(Thr) specific for ACY codons, using S-adenosyl-L-methionine (AdoMet) as a methyl donor [1]. This modification seems to be involved in ensuring efficient decoding of ACY and enhancing the attenuation activity of the thr operon, suggesting its importance in translational processes [1].
In an E. coli ΔtrmO strain, m(6)t(6)A was converted to cyclic t(6)A (ct(6)A), indicating that t(6)A is a common precursor for both m(6)t(6)A and ct(6)A [1]. Also, a human homolog, TRMO, was identified, suggesting a conserved role in fine-tuning decoding from bacteria to mammals [1]. Additionally, in a study on differentiated thyroid carcinoma, TRMO was identified as one of the genes at the 9q22 locus associated with the disease, hinting at its potential role in thyroid carcinoma susceptibility [2].
In conclusion, TrmO is crucial for tRNA methylation and translational efficiency. Its role in organisms from bacteria to mammals, as well as its potential association with differentiated thyroid carcinoma, as revealed through genetic model-based research, highlights its significance in both basic biological processes and disease-related studies.
References:
1. Kimura, Satoshi, Miyauchi, Kenjyo, Ikeuchi, Yoshiho, Crécy-Lagard, Valérie de, Suzuki, Tsutomu. 2014. Discovery of the β-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs. In Nucleic acids research, 42, 9350-65. doi:10.1093/nar/gku618. https://pubmed.ncbi.nlm.nih.gov/25063302/
2. Kulkarni, Om, Sugier, Pierre-Emmanuel, Guibon, Julie, Truong, Thérèse, Lesueur, Fabienne. 2021. Gene network and biological pathways associated with susceptibility to differentiated thyroid carcinoma. In Scientific reports, 11, 8932. doi:10.1038/s41598-021-88253-0. https://pubmed.ncbi.nlm.nih.gov/33903625/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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