C57BL/6JCya-Vcam1em1/Cya
Common Name:
Vcam1-KO
Product ID:
S-KO-15784
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Vcam1-KO
Strain ID
KOCMP-22329-Vcam1-B6J-VA
Gene Name
Product ID
S-KO-15784
Gene Alias
CD106; Vcam-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vcam1em1/Cya mice (Catalog S-KO-15784) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029574
NCBI RefSeq
NM_011693
Target Region
Exon 1
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Vcam1, known as Vascular cell adhesion molecule 1, belongs to the immunoglobulin superfamily of cell adhesion molecules. It plays a crucial role in mediating the strong adherence of leukocytes to endothelial cells, regulating inflammation and leukocyte migration. It is involved in multiple biological processes such as lymphocyte recirculation and immune response, and is associated with numerous diseases including cancer, atherosclerosis, and asthma [3].
In gastric cancer, lactate in the tumor microenvironment promotes H3K18 lactylation, leading to transcriptional activation of Vcam1, which then activates AKT-mTOR signaling to enhance cell proliferation and migration. Also, Vcam1 upregulates CXCL1 expression via AKT-mTOR pathway, facilitating the recruitment of mesenchymal stem cells, immunesuppression, and cancer progression [1]. In ovarian cancer, the VLA4/VCAM1 pathway in macrophages and endothelial cells is involved in regulating vascular permeability and ascites development. Downregulating VLA4 or Vcam1 enhances cell adhesion and vascular integrity, abrogating ascites formation [2]. In sickle cell anemia, certain Vcam1 promoter haplotypes increase its expression, affecting systemic vasculopathy risk [4]. In liver fibrosis, Vcam1 expressed by liver sinusoidal endothelial cells promotes capillarization and fibrosis. Blocking Vcam1 can restore the phenotype of differentiated endothelial cells and reduce fibrosis-related markers in hepatic stellate cells [5]. In chronic cerebral hypoperfusion, increased Vcam1 expression on brain endothelial cells drives blood-brain-barrier impairment, and blocking Vcam1 activation alleviates this effect [6].
In conclusion, Vcam1 is essential for leukocyte adhesion, inflammation regulation, and immune response. Through gene-knockout or conditional-knockout mouse models and other functional studies, its role in various diseases has been revealed. These findings provide potential therapeutic targets for diseases such as cancer, ovarian cancer with ascites, sickle cell anemia, liver fibrosis, and chronic cerebral hypoperfusion-related neurodegenerative diseases.
References:
1. Zhao, Yupeng, Jiang, Jiang, Zhou, Peng, Li, Ranran, Xia, Jiazeng. 2024. H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis. In Biochemical pharmacology, 222, 116120. doi:10.1016/j.bcp.2024.116120. https://pubmed.ncbi.nlm.nih.gov/38461905/
2. Zhang, Shibo, Xie, Bingfan, Wang, Lijie, Liu, Changqing, He, Huanhuan. . Macrophage-mediated vascular permeability via VLA4/VCAM1 pathway dictates ascites development in ovarian cancer. In The Journal of clinical investigation, 131, . doi:10.1172/JCI140315. https://pubmed.ncbi.nlm.nih.gov/33295887/
3. Singh, Varinder, Kaur, Rupinder, Kumari, Pratima, Pasricha, Chirag, Singh, Ravinder. 2023. ICAM-1 and VCAM-1: Gatekeepers in various inflammatory and cardiovascular disorders. In Clinica chimica acta; international journal of clinical chemistry, 548, 117487. doi:10.1016/j.cca.2023.117487. https://pubmed.ncbi.nlm.nih.gov/37442359/
4. Silva, Marisa, Coelho, Andreia, Vargas, Sofia, Faustino, Paula. 2021. VCAM1, HMOX1 and NOS3 differential endothelial expression may impact sickle cell anemia vasculopathy. In Blood cells, molecules & diseases, 93, 102639. doi:10.1016/j.bcmd.2021.102639. https://pubmed.ncbi.nlm.nih.gov/34999313/
5. Guo, Qianqian, Furuta, Kunimaro, Islam, Shahidul, Hirsova, Petra, Ibrahim, Samar H. 2022. Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis. In Frontiers in immunology, 13, 983255. doi:10.3389/fimmu.2022.983255. https://pubmed.ncbi.nlm.nih.gov/36091042/
6. Zhang, Huiwen, Shang, Junkui, Li, Wei, Gao, Dandan, Zhang, Jiewen. 2024. Increased Expression of VCAM1 on Brain Endothelial Cells Drives Blood-Brain Barrier Impairment Following Chronic Cerebral Hypoperfusion. In ACS chemical neuroscience, 15, 2028-2041. doi:10.1021/acschemneuro.4c00039. https://pubmed.ncbi.nlm.nih.gov/38710594/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen