Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-B4galt1em1/Cya
Common Name:
B4galt1-KO
Product ID:
S-KO-16164
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
B4galt1-KO
Strain ID
KOCMP-14595-B4galt1-B6N-VC
Gene Name
B4galt1
Product ID
S-KO-16164
Gene Alias
B-1; 4-GalT; 4-GalT1; GalT; Ggtb; Ggtb2; b4Gal-T1; beta-1; beta4Gal-T1
Background
C57BL/6NCya
NCBI ID
14595
Modification
Conventional knockout
Chromosome
4
Phenotype
MGI:95705
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-B4galt1em1/Cya mice (Catalog S-KO-16164) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030121
NCBI RefSeq
NM_022305
Target Region
Exon 1
Size of Effective Region
~0.6 kb
Detailed Document
Click here to download >>
Overview of Gene Research
B4galt1, also known as beta-1,4-galactosyltransferase1, is a key gene involved in N-glycan biosynthesis. N-glycan biosynthesis is a crucial process that impacts the structure and function of many proteins, thus influencing various biological pathways and processes [1].

In lung adenocarcinoma, B4galt1 was highly expressed in invasive adenocarcinoma samples. Inhibition of B4galt1 in in vivo models increased CD8 + T-cell abundance and activity, enhancing the antitumour immunity of anti-PD-1 therapy. B4galt1 regulated LUAD cell proliferation and invasion both in vitro and in vivo, and was related to the impaired antitumour capacity of CD8 + T cells. Mechanistically, it directly mediates the N-linked glycosylation of PD-L1 protein, preventing its degradation, and stabilizes the TAZ protein via glycosylation, activating CD274 transcriptionally, leading to immune escape [1].

In hepatocellular carcinoma, decreased B4galt1 enhanced cell migration and invasion in vitro and promoted lung metastasis in NOD/SCID mice. B4galt1 downregulation altered N-glycosylation and enhanced the laminin-binding activity of integrin α6 and integrin β1 to promote invasiveness [2].

In glioblastoma, B4galt1 knockdown by lentivirus inhibited glioblastoma development in vitro and in vivo, increased apoptosis and autophagy [3].

In pancreatic ductal adenocarcinomas, high B4galt1 expression correlated with poor survival, and its depletion rescued the response of chemoresistant cells to gemcitabine in an orthotopic model. Mechanistically, elevated p65 activity up-regulated B4galt1, which then interacted with and stabilized CDK11p110 via N-linked glycosylation to promote cancer progression and chemoresistance [4].

In acute myeloid leukemia, B4galt1 was overexpressed, and silencing it significantly promoted apoptosis [5].

In conclusion, B4galt1 is essential in N-glycan biosynthesis and significantly impacts various disease conditions. Gene-knockout or knockdown models in different cancers such as lung adenocarcinoma, hepatocellular carcinoma, glioblastoma, pancreatic ductal adenocarcinomas, and acute myeloid leukemia have revealed its role in processes like cell proliferation, invasion, immune escape, apoptosis, and chemoresistance, offering potential therapeutic targets for these diseases.

References:

1. Cui, Yanan, Li, Jun, Zhang, Pengpeng, Zhang, Erbao, Guo, Renhua. 2023. B4GALT1 promotes immune escape by regulating the expression of PD-L1 at multiple levels in lung adenocarcinoma. In Journal of experimental & clinical cancer research : CR, 42, 146. doi:10.1186/s13046-023-02711-3. https://pubmed.ncbi.nlm.nih.gov/37303063/

2. Chen, Po-Da, Liao, Ying-Yu, Cheng, Yu-Chia, Wu, Yao-Ming, Huang, Min-Chuan. 2023. Decreased B4GALT1 promotes hepatocellular carcinoma cell invasiveness by regulating the laminin-integrin pathway. In Oncogenesis, 12, 49. doi:10.1038/s41389-023-00494-y. https://pubmed.ncbi.nlm.nih.gov/37907465/

3. Wang, Pu, Li, Xiaolong, Xie, Yuan. . B4GalT1 Regulates Apoptosis and Autophagy of Glioblastoma In Vitro and In Vivo. In Technology in cancer research & treatment, 19, 1533033820980104. doi:10.1177/1533033820980104. https://pubmed.ncbi.nlm.nih.gov/33287670/

4. Chen, Yitian, Su, Liangping, Huang, Cheng, Liu, Chao, Wong, Ping-Pui. 2020. Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. In Cancer letters, 500, 228-243. doi:10.1016/j.canlet.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33309857/

5. Ren, Zhihong, Huang, Xiaoyu, Lv, Qing, Wang, Fanping, Wang, Mingyong. 2022. High expression of B4GALT1 is associated with poor prognosis in acute myeloid leukemia. In Frontiers in genetics, 13, 882004. doi:10.3389/fgene.2022.882004. https://pubmed.ncbi.nlm.nih.gov/36568388/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest